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Universal USER Cassette |
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Prices are in US dollars and valid only for US orders.
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Description:
The Universal USER Cassette enables the construction of USER compatible vectors by ligation of an unphosphorylated double-stranded oligonucleotide cassette into any blunt-ended phosphorylated vector. Once the cassette is ligated into the vector of choice, this vector is digested with nicking enzyme Nt.BbvCI and the restriction enzyme XbaI which creates DNA overhangs compatible with the USER processed PCR product described in the USER Friendly PCR Cloning Kit (NEB #E5500).
Reagents Supplied:
Preannealed, unphosphorylated oligonucleotides
Reaction & Storage Conditions
Concentration:
2.5 pmol/μl
Storage Temperature:
-20°C
Notes
General notes:- Reagents Supplied: Preannealed, unphosphorylated oligonucleotides (50 pmol, 2.5 pmol/µl) in 20 µl of 10 mM Tris-HCl, 1 mM EDTA (pH 8.0).
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
The DNA sequence of the cassette was confirmed by DNA sequencing after cloning the Universal USER Cassette into LITMUS 38 digested with StuI. Using either the High Concentration T4 DNA Ligase Reaction protocol or Quick Ligation Reaction protocol outlined below, 20-40% of transformants had the cassette inserted into the vector.
Companion Products
USER Enzyme
USER Friendly Cloning Kit
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