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FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE M13mp18 RF I DNA M13mp19 RF I DNA

Home > Products > Nucleic Acids > Phage DNAs > M13mp18 Single-stranded DNA M13mp18 Single-stranded DNA Catalog # Size Concentration Price Qty   N4040S 10 μg 250 μg/ml $30.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: The single-stranded viral DNA is isolated from M13mp18. M13mp18 is a M13 lac phage vector which contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the gene encoding β-Galactosidase.This DNA preparation is useful as a standard and has been tested as a template in the dideoxy-nucleotide termination method of sequencing DNA. Source: M13mp18 phage is propagated in E. coli K12 JM101. The phage is purified by polyethylene glycol precipitation. The single-stranded DNA is then extracted with phenol. Concentration: 250 μg/ml Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 7.5 @ 25°C Storage Temperature: -20°C References Messing, J. (1983) R. Wu (Eds.), Methods Enzymol., 101, pp. 20. San Diego: Academic Press. Companion Products M13mp18 RF I DNA M13mp19 RF I DNA