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NIH 3T3 Mouse Genomic DNA |
 | | | | NIH 3T3 Mouse Genomic DNA | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
NIH 3T3 (mouse embryonic fibroblast cell line) genomic DNA.
Source:
NIH 3T3 (mouse embryonic fibroblast) cells are grown to confluency in DMEM plus 10% fetal bovine serum. Genomic DNA was isolated by a standard genomic purification protocol (1), phenol extracted and equilibrated to 10 mM Tris-HCl (pH 7.5) and 1 mM EDTA.
Applications:- A control for Methylation-Specific PCR (MSP) (2), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (Ms-SNuPE), Combined Bisulfite Restriciton Analysis (COBRA), Bisulfite treatment and PCR-Single-Strand Conformation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS).
- PCR, SNP Analysis, Southern Blotting
- Genomic DNA library construction
Reaction & Storage Conditions
Concentration:
100 μg/ml
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C
Storage Temperature:
-20°C
Avoid repeated freeze/thaw cycles.
Notes
General notes:- A260/280 Ratio: 1.90
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
Purified free of contaminating proteins and RNA.
References
- Sambrook, J. and Russell, D. (2001) Molecular Cloning: A Laboratory Manual, (3rd ed.), (pp. 6.4-6.12). Cold Spring Harbor: Cold Spring Ha.
- Herman,J. G. and Baylin, S. B. (1996) U.S. Patent No, 5,786,146, Johns Hopkins University School of Medicine.
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