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FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE K. lactis GG799 Competent Cells K. lactis Protein Expression Kit Integration Primer Set pKLAC1 Vector

Home > Products > Nucleic Acids > K. lactis Vectors > pKLAC1-malE Control Plasmid pKLAC1-malE Control Plasmid Catalog # Size Concentration Price Qty   N3741S 20 μg 1,000 μg/ml $275.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Expression of the fusion gene is under the control of the strong K.lactis PLAC4-PBI promoter K.lactis strain GG799 can be used as an expression host Ampicillin resistance for propagation in E.coli Fungal acetamidase selectable marker for integration into the K.lactis genome Description: pKLAC1-malE is a control plasmid for the K. lactis Expression Kit (NEB #E1000S). The backbone integrative expression vector, pKLAC1, directs high-level expression of recombinant protein from the yeast Kluyveromyces lactis. Vector pKLAC1-malE carries the E.coli malE gene, encoding the maltose binding protein (MBP). pKLAC1-malE can be used to test plasmid transformation, cell culture, and protein production procedures. A typical K. lactis GG799 strain containing integrated pKLAC1-malE will produce > 25 mg/L of MBP (Figure 1). Figure 1: SDS-polyacrylamide gel electrophoresis of secreted recombinant maltose binding protein (MBP). (A) Detection of secreted MBP by Coomassie staining. Lane 1: Protein Molecular Weight Markers. Lane 2: spent culture medium (15 µl) from wild-type K. lactis cells. Lane 3: spent culture medium (15 µl) from K. lactis cells harboring integrated pKLAC1-malE. (B) Detection of secreted MBP by Western analysis using a polyclonal anti-MBP antibody (NEB #E8031S; 1:2000 dilution) and an anti-rabbit IgG-HRP secondary antibody (1:2000 dilution). Lane 1: culture medium from K. lactis cells (15 µl). Lane 2: culture medium (15 µl) from K. lactis cells containing integrated pKLAC1-malE. Source: pKLAC1-malE is isolated from E. coli strain ER2268 by a standard plasmid purification procedure. Concentration: 1,000 μg/ml Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 7.5 @ 25°C Storage Temperature: -20°C References Colussi, P.A. and Taron, C.H., unpublished observations. Companion Products K. lactis GG799 Competent Cells K. lactis Protein Expression Kit Integration Primer Set pKLAC1 Vector Legal Research Use Assurance: The vector pKLAC1-malE is a component of an expression system that was developed from basic research at New England Biolabs, Inc. and DSM Biologics Company B.V. The buyer/user has a non-exclusive sublicense to use this system or any component thereof, including vector pKLAC1-malE, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V.