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FAQs for LITMUS 28i Vector FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE HiScribe RNAi Transcription Kit LITMUS 28/38 Forward Sequencing Primer (21-mer) LITMUS 28/38 Reverse Sequencing Primer (21-mer) LITMUS 38i Vector

Home > Products > Nucleic Acids > LITMUS Transcription Vectors > LITMUS 28i Vector LITMUS 28i Vector Catalog # Size Concentration Price Qty   N3528S 20 μg 500 μg/ml $79.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Extensive set of restriction sites in polylinker, many with unique 4-base overhangs Blue/white section Opposing T7promoters for making RNA transcripts in either direction or double-stranded RNA using the HiScribe RNAi Transcription Kit (NEB #E2000S) Description: LITMUS 28i is a multi-purpose cloning/in vitro transcription phagemid vector. The molecule is a small, double-stranded circle 2,823 base pairs in length (molecular weight =1.8 x 106 daltons). Source: LITMUS 28i is isolated from E. coli ER2738 by a standard plasmid purification procedure. Advantages: Small (< 3 kb), high copy number. Ampicillin resistance. Single-stranded (M13) DNA replication origin. Compatible with pUC/M13 sequencing primers as well as LITMUS sequencing primers. Concentration: 500 μg/ml Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C Storage Temperature: -20°C Notes General notes: Appropriate strains of E. coli containing the LITMUS 28i plasmid form blue colonies on X-gal plates; when a fragment is inserted in the polylinker, the colonies are white. Blue/white selection is best achieved by plating on a rich media (e.g. LB) supplemented with X-gal (40 µg/ml) and IPTG (0.1 mM). FAQs How homogeneous is the DNA/ how much is supercoiled? Are the plasmids cesium purified? What primers should I use to sequence an insert (pUC19, pNEB193, LITMUS)? Enzymes in parentheses ( ) leave 3' extension and should not be used for template linearization. Companion Products HiScribe RNAi Transcription Kit LITMUS 28/38 Forward Sequencing Primer (21-mer) LITMUS 28/38 Reverse Sequencing Primer (21-mer) LITMUS 38i Vector Legal Patents: New England Biolabs, Inc.: U.S. 5,691,140 Research Use Assurance: The Buyer/User has a non-exclusive license to use this vector for RESEARCH PURPOSES ONLY.* Commercial use of the LITMUS Vectors may require a license from New England Biolabs. *Use of this vector for RNA interference may require a license from the Carnegie Institution of Washington. For further information, contact the Director of Administration and Finance at the Carnegie Institute of Washington at 1530 P Street, N.W., Washington D.C., 20005-1910. Tel: 202-939-1118.