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Nucleic Acids >
DNA Markers/Ladders >
TriDye™ 1 kb DNA Ladder |
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Prices are in US dollars and valid only for US orders.
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Description:
TriDye™ 1 kb DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.
The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases. The 3.0 kb fragment has increased intensity to serve as a reference band.
TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.
1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/lane.
Effective Size Range: 500 bp to 10,002 bp
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| 1 | | 10,002 | | 42 | | |
| 2 | | 8,001 | | 42 | | |
| 3 | | 6,001 | | 50 | | |
| 4 | | 5,001 | | 42 | | |
| 5 | | 4,001 | | 33 | | |
| 6 | | 3,001 | | 125 | | |
| 7 | | 2,000 | | 48 | | |
| 8 | | 1,500 | | 36 | | |
| 9 | | 1,000 | | 42 | | |
| 10a | | 517 | | 42 | | |
| 10b | | 500 | | 42 | | |
Concentration:
50 μg/ml
Recommended Load: 10 μl
Storage Conditions
Storage Conditions:
0.006% Xylene Cyanol FF
10 mM Tris-HCl
10 mM EDTA
10% Glycerol
0.006% Bromophenol Blue
0.06% Orange G
pH 7.9 @ 25°C
Storage Temperature:
4°C
Notes
General notes:- TriDye 1 kb DNA Ladder is stable for at least 6 months at 25°C.
- For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.
Usage notes:- We recommend loading 10 μl (0.5 μg) of TriDye 1kb DNA Ladder for gel lane.
- The TriDye 1 kb DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
FAQs
- What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
- Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
- How can I quantify the amount of DNA in each band of a marker?
References
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual (2nd Ed.), 10.51-10.67.
Companion Products
1 kb DNA Ladder
100 bp DNA Ladder
2-Log DNA Ladder (0.1–10.0 kb)
PCR Marker
Quick-Load® 1 kb DNA Ladder
Quick-Load® 100 bp DNA Ladder
Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb)
TriDye™ 100 bp DNA Ladder
TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)
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