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FAQs for TriDye™ 100 bp DNA Ladder FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE 1 kb DNA Ladder 100 bp DNA Ladder 2-Log DNA Ladder (0.1–10.0 kb) PCR Marker Quick-Load® 1 kb DNA Ladder Quick-Load® 100 bp DNA Ladder Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb) TriDye™ 1 kb DNA Ladder TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

Home > Products > Nucleic Acids > DNA Markers/Ladders > TriDye™ 100 bp DNA Ladder TriDye™ 100 bp DNA Ladder Catalog # Size Concentration Price Qty   N3271S 125 gel lanes 50 μg/ml $83.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Description: TriDye™ 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. The DNA ladder consists of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points. TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change. 100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 µg/lane Effective Size Range: 100 bp to 1,517 bp Source: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Fragment Size (bp) Mass (ng) 1 1,517 45 2 1,200 35 3 1,000 95 4 900 27 5 800 24 6 700 21 7 600 18 8 517 97 8 500 97 9 400 38 10 300 29 11 200 25 12 100 48 Concentration: 50 μg/ml Recommended Load: 10 μl Storage Conditions Storage Conditions: 0.006% Xylene Cyanol FF 10 mM Tris-HCl 10 mM EDTA 10% Glycerol 0.006% Bromophenol Blue 0.06% Orange G pH 7.9 @ 25°C Storage Temperature: 4°C Notes General notes: TriDye 100 bp DNA Ladder is stable for at least 6 months at 25°C For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. Usage notes: The TriDye 100 bp DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size. We recommend loading 10 μl (1.0 μg) of TriDye 100 bp DNA Ladder per gel lane. FAQs What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment? Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands? How can I quantify the amount of DNA in each band of a marker? References Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual (2nd Ed.), 10.51-10.67. Companion Products 1 kb DNA Ladder 100 bp DNA Ladder 2-Log DNA Ladder (0.1–10.0 kb) PCR Marker Quick-Load® 1 kb DNA Ladder Quick-Load® 100 bp DNA Ladder Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb) TriDye™ 1 kb DNA Ladder TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)