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FAQs for 50 bp DNA Ladder Protocols for 50 bp DNA Ladder FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE Gel Loading Dye, Blue (6X)

Home > Products > Nucleic Acids > DNA Markers/Ladders > 50 bp DNA Ladder 50 bp DNA Ladder Catalog # Size Concentration Price Qty   N3236L 500 gel lanes 1,000 μg/ml $244.00 N3236S 100 gel lanes 1,000 μg/ml $61.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Description: A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose and polyacrylamide gel electrophoresis. The digested DNA includes fragments ranging from 50-1,350 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points. 50 bp DNA Ladder visualized by ethidium bromide staining on a 1.8% TBE agarose gel. Mass values are for 1 µg/lane. Effective Size Range: 50 bp to 1,350 bp Source: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Reagents Supplied: Gel Loading Dye, Blue (6X) Fragment Size (bp) Mass (ng) 1 1,350 103 2 916 70 3 766 58 4 700 54 5 650 50 6 600 46 7 550 42 8 500 76 9 450 34 10 400 31 11 350 27 12 300 46 13 250 57 14 200 107 15 150 46 16 100 69 17 50 84 Concentration: 1,000 μg/ml Recommended Load: 1 μg Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA Storage Temperature: -20°C For long term storage (>30 days), store at -20°C. Notes General notes: All ends have 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (NEB #M0201) or filled-in using DNA Polymerase I, Klenow Fragment (NEB #M0210) (1). Use α-[32P] dCTP or α-[32P] dGTP for the fill-in reaction. 50 bp DNA Ladder is stable for at least 3 months at 4°C. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may be denatured if diluted in dH2O. 1X Gel Loading Dye, Blue: 2.5% Ficoll-400 11 mM EDTA 3.3 mM Tris-HCl (pH 8.0@25°C) 0.017% SDS 0.015% bromophenol blue Due to the limitations of the acrylamide gel technology, one or two extra bands may be visible on the DNA ladders when run on a polyacrylamide gel. Usage notes: We recommend loading 1.0 µg of 50 bp DNA Ladder diluted in sample buffer. The 50 bp DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size. The 50 bp DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size. FAQs Why are there extra bands visible on polyacrylamide gels? Why is the separation of the lower bands incomplete? What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment? Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands? How can I quantify the amount of DNA in each band of a marker? References Sambrook, J., Fritsch, E.F. and Maniatis, T.  (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), 10.51-10.67. Reagents Sold Separately Gel Loading Dye, Blue (6X)