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Nucleic Acids >
DNA Markers/Ladders >
PCR Marker |
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Prices are in US dollars and valid only for US orders.
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Description:
The PCR Marker consists of a proprietary plasmid that is digested to completion with appropriate restriction enzymes to yield 5 double-stranded DNA bands suitable for use as molecular weight standards for agarose and acrylamide gel electrophoresis. The digested DNA includes fragments ranging from 50-766 base pairs. The approximate mass of DNA in each of the bands is provided (assuming a 0.3 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
PCR Marker visualized by ethidium bromide staining on a 1.8% TBE Agarose Gel. Mass values are for 0.3 μg/lane.
Effective Size Range: 50 bp to 766 bp
Source:
Double-stranded DNA is digested to completion with the appropriate restriction enzymes, phenol extracted and equilibrated to 10 mM Tris-HCl (ph 8.0) and 1 mM EDTA.
Reagents Supplied:
Gel Loading Dye, Blue (6X)
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| 1 | | 766 | | 62 | | |
| 2 | | 500 | | 40 | | |
| 3 | | 300 | | 48 | | |
| 4 | | 150 | | 61 | | |
| 5 | | 50 | | 89 | | |
Concentration:
300 μg/ml
Recommended Load: 0.3 μg
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
Storage Temperature:
4°C
For long term storage (>30 days), store at -20°C.
Notes
General notes:- PCR Marker is stable for at least 3 months at 4°C.
- For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.
- All ends have 5' overhangs that can be labeled using T4 Polynucleotide Kinase (NEB #M0201) or filled-in using DNA Polymerase I, Klenow Fragment (NEB #M0210) (1). Use α-[32P] dCTP or α-[32P] dGTP for the fill-in reaction.
- 1X Gel Loading Dye, Blue:
2.5% Ficoll-400
11 mM EDTA
3.3 mM Tris-HCL (pH 8.0@ 25°C)
0.017% SDS
0.015% bromophenol blue
Usage notes:- We recommend loading 0.3 μg of PCR Marker diluted in sample buffer.
- This marker was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
FAQs
- What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
- How can I quantify the amount of DNA in each band of a marker?
Reagents Sold Separately
Gel Loading Dye, Blue (6X)
Companion Products
1 kb DNA Ladder
100 bp DNA Ladder
2-Log DNA Ladder (0.1–10.0 kb)
TriDye™ 1 kb DNA Ladder
TriDye™ 100 bp DNA Ladder
TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)
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