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FAQs for Low Molecular Weight DNA Ladder Protocols for Low Molecular Weight DNA Ladder FAQs for Markers and Ladders (DNA/RNA/Protein) Technical Reference for Markers and Ladders (DNA/RNA/Protein) Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE Gel Loading Dye, Blue (6X)

Home > Products > Markers and Ladders (DNA/RNA/Protein) > DNA Markers/Ladders > Low Molecular Weight DNA Ladder Low Molecular Weight DNA Ladder Catalog # Size Concentration Price Qty   N3233L 500 gel lanes 500 μg/ml $244.00 N3233S 100 gel lanes 500 μg/ml $61.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A proprietary plasmid is digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for both agarose and polyacrylamide gel electrophoresis. This digested DNA includes fragments ranging from 25-766 base pairs. The 200 base pair band has increased intensity to serve as a reference point. LMW DNA Ladder visualized by ethidium bromide staining on a 1.8% TBE agarose gel. Mass values are for 0.5 µg/lane. Effective Size Range: 25 bp to 766 bp Source: Double-stranded DNA is digested to completion with the appropriate restriction enzymes, phenol extracted, ethanol precipitated and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Reagents Supplied: Gel Loading Dye, Blue (6X) Fragment Size (bp) Mass (ng) 1 766 42 2 500 27 3 350 20 4 300 33 5 250 27 6 200 110 7 150 33 8 100 43 9 75 58 10 50 63 11 25 43 Concentration: 500 μg/ml Recommended Load: 0.5 μg Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA Storage Temperature: -20°C For long term storage (>30 days), store at -20°C. Notes General notes: All ends have 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (NEB #M0201) or filled-in using DNA Polymerase I, Klenow Fragment (NEB #M0210) (1). Use α-[32P] dCTP or α-[32P] dGTP for the fill-in reaction. DNA ladders are stable for at least 3 months at 4°C. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O. 1X Gel Loading Dye, Blue: 2.5% Ficoll-400 11 mM EDTA 3.3 mM Tris-HCL (pH 8.0@25°C)  0.017% SDS 0.015% bromophenol blue Due to the limitations of the acrylamide gel technology, one or two extra bands may be visible on the DNA ladders when run on a polyacrylamide gel. Usage notes: We recommend loading 0.5 µg of the Low Molecular Weight DNA Ladder diluted in sample buffer. This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size. FAQs Why are there extra bands visible on polyacrylamide gels? Why is the separation of the lower bands incomplete? What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment? Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands? How can I quantify the amount of DNA in each band of a marker? Protocols Protocols for Low Molecular Weight DNA Ladder References Sambrook, J., Fritsch, E.F. and Maniatis, T.  (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), 10.51-10.67. Reagents Sold Separately Gel Loading Dye, Blue (6X) New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201