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2-Log DNA Ladder (0.1–10.0 kb) |
 | | | | 2-Log DNA Ladder (0.1–10.0 kb) | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference points. The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane.
Effective Size Range: 100 bp to 10 kb
Source:
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Reagents Supplied:
Gel Loading Dye, Blue (6X)
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| 1 | | 10,002 | | 40 | | |
| 2 | | 8,001 | | 40 | | |
| 3 | | 6,001 | | 48 | | |
| 4 | | 5,001 | | 40 | | |
| 5 | | 4,001 | | 32 | | |
| 6 | | 3,001 | | 120 | | |
| 7 | | 2,017 | | 40 | | |
| 8 | | 1,517 | | 57 | | |
| 9 | | 1,200 | | 45 | | |
| 10 | | 1,000 | | 122 | | |
| 11 | | 900 | | 34 | | |
| 12 | | 800 | | 31 | | |
| 13 | | 700 | | 27 | | |
| 14 | | 600 | | 23 | | |
| 15a | | 517 | | 124 | | |
| 15b | | 500 | | 124 | | |
| 16 | | 400 | | 49 | | |
| 17 | | 300 | | 37 | | |
| 18 | | 200 | | 32 | | |
| 19 | | 100 | | 61 | | |
Concentration:
1,000 μg/ml
Recommended Load: 1 μg
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
Storage Temperature:
-20°C
For long term storage (>30 days), store at -20°C.
Notes
General notes:- All fragments have 4-base, 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (NEB #M0201) or filled-in using DNA Polymerase I, Klenow Fragment (NEB #M0210) (1). Use α-[32P] dATP or α-[32P] dTTP for the fill-in reaction.
- 2-Log DNA Ladder is stable for at least 3 months at 4°C.
- For long term storae, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20
- 1X Gel Loading Dye, Blue:
2.5% Ficoll-400
11 mM EDTA
3.3 mM Tris-HCl (pH 8.0@25°C)
0.017% SDS
0.015% bromophenol blue - When DNA ladders are run on an acrylamide gel, some separation of the reference bands may be observed due to variations in the nucleic acid composition of the DNA molecules within that band.
Usage notes:- This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
- We recommend loading 1 μg of the 2-Log DNA Ladder diluted in sample buffer.
FAQs
- Why are there extra bands visible on polyacrylamide gels?
- What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
- Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
- How can I quantify the amount of DNA in each band of a marker?
References
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual (2nd Ed.), 10.51-10.67.
Reagents Sold Separately
Gel Loading Dye, Blue (6X)
Companion Products
1 kb DNA Ladder
100 bp DNA Ladder
Biotinylated 2-Log DNA Ladder (0.1-10 kb)
HiScribe RNAi Transcription Kit
PCR Marker
Quick-Load® 1 kb DNA Ladder
Quick-Load® 100 bp DNA Ladder
Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb)
TriDye™ 1 kb DNA Ladder
TriDye™ 100 bp DNA Ladder
TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)
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