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FAQs for pUC19 Vector FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE pBR322 Vector pNEB193 Vector

Home > Products > Nucleic Acids > Additional Cloning Vectors > pUC19 Vector pUC19 Vector Catalog # Size Concentration Price Qty   N3041L 250 μg 1,000 μg/ml $244.00 N3041S 50 μg 1,000 μg/ml $61.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: pUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1). GenBank Assession Number: L09137 Source: pUC19 is isolated from E. coli ER2272 (dam+ dcm+ EcoK M-) by a standard plasmid purification procedure. Vector Properties General Utility: Cloning Selectable Markers: Ampicillin Origin of Replication: ColE1 Copy Number: High (100+) Molecular Weight: Theoretical: 1,750,000 daltons Concentration: 1,000 μg/ml Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C Storage Temperature: -20°C FAQs How homogeneous is the DNA/ how much is supercoiled? Are the plasmids cesium purified? What primers should I use to sequence an insert (pUC19, pNEB193, LITMUS)? References Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Gene, 33, 103-119. Companion Products pBR322 Vector pNEB193 Vector