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FAQs for pBR322 Vector FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE pNEB193 Vector pUC19 Vector

Home > Products > Nucleic Acids > Additional Cloning Vectors > pBR322 Vector pBR322 Vector Catalog # Size Concentration Price Qty   N3033L 250 μg 1,000 μg/ml $244.00 N3033S 50 μg 1,000 μg/ml $61.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight is 2.83 x 106 daltons. *Sequencing data from Watson (confirmed at New England Biolabs, Inc.) has shown pBR322 to be 4,361 base pairs, not 4,363 base pairs as previously reported. Source: pBR322 is isolated from E. coli ER2272 (dam +dcm + EcoK M-) by a standard plasmid purification procedure. Concentration: 1,000 μg/ml Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C Storage Temperature: -20°C FAQs How homogeneous is the DNA/ how much is supercoiled? Are the plasmids cesium purified? References Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heynecker, H.L. and Boyer, H.W. (1977) Gene, 2, 95-113. Watson, N. (1988) Gene, 70, 399-403. Companion Products pNEB193 Vector pUC19 Vector