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FAQs for pBR322 DNA-BstNI Digest Protocols for pBR322 DNA-BstNI Digest FAQs for Markers and Ladders (DNA/RNA/Protein) Technical Reference for Markers and Ladders (DNA/RNA/Protein) Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE Gel Loading Dye, Blue (6X) 1 kb DNA Ladder 100 bp DNA Ladder 2-Log DNA Ladder (0.1–10.0 kb) PCR Marker TriDye™ 1 kb DNA Ladder TriDye™ 100 bp DNA Ladder TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

Home > Products > Markers and Ladders (DNA/RNA/Protein) > Conventional Markers > pBR322 DNA-BstNI Digest pBR322 DNA-BstNI Digest Catalog # Size Concentration Price Qty   N3031L 250 gel lanes 1,000 μg/ml $252.00 N3031S 50 gel lanes 1,000 μg/ml $63.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: The BstNI Digest of pBR322 DNA yields 6 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1,2). pBR322 DNA-BstNI Digest visualized by ethidium bromide staining. 1.4% agarose gel. Effective Size Range: 13 bp to 1,857 bp Source: The double-stranded DNA is digested to completion with BstNI, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Reagents Supplied: Gel Loading Dye, Blue (6X) Fragment Size (bp) 1 1,857 2 1,058 3 929 4 383 5 121 6 13 Concentration: 1,000 μg/ml Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C Storage Temperature: -20°C Notes General notes: For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O. 1X Gel Loading Dye, Blue: 2.5% Ficoll-400 11 mM EDTA 3.3 mM Tris-HCl (pH 8.0@2.5°C) 0.017% SDS 0.015% bromophenol blue FAQs Why is the separation of the lower bands incomplete? What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment? How can I quantify the amount of DNA in each band of a marker? Protocols Protocols for pBR322 DNA-BstNI Digest References Sutcliffe, J.G. (1978) Cold Spring Harbor Symp. Quant. Bio., 43, 77-90. Peden, K.W.C. (1983) Gene, 22, 277-280. Reagents Sold Separately Gel Loading Dye, Blue (6X) Companion Products 1 kb DNA Ladder 100 bp DNA Ladder 2-Log DNA Ladder (0.1–10.0 kb) PCR Marker TriDye™ 1 kb DNA Ladder TriDye™ 100 bp DNA Ladder TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb) New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201