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Prices are in US dollars and valid only for US orders.
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Description:
The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI. The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing. These fragments are best separated by Pulsed Field Gel Electrophoresis.The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
PFGE separation of 0.5 µg of Lambda Mono Cut Mix. 1% agarose gel, 0.5X TBE, 6 V/cm, 15°C for 20 hours. Switch times ramped from 0.5-1.5 seconds.
Effective Size Range: 1,503 bp to 48,502 bp
Source:
The phage is isolated from the heat- inducible lysogen E. coli λ c1857 S7 and then isolated from the purified phage by phenol extraction and dialyzed. The double-stranded DNA is then digested to completion with the appropriate enzyme, phenol extracted and dialyzed against 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Reagents Supplied:
Gel Loading Dye, Blue (6X)
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| 1 | | 48,502 | | 100 | | |
| 2 | | 38,416 | | 79 | | |
| 3 | | 33,498 | | 69 | | |
| 4 | | 29,946 | | 62 | | |
| 5 | | 24,508 | | 51 | | |
| 6 | | 23,994 | | 49 | | |
| 7 | | 17,053 | | 35 | | |
| 8 | | 15,004 | | 31 | | |
| 9 | | 10,086 | | 21 | | |
| 10 | | 1,503 | | 3 | | |
Concentration:
500 μg/ml
Recommended Load: 0.5 μg
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
Storage Temperature:
-20°C
Notes
Usage notes:- These fragments are best separated by Pulsed Field Gel Electrophoresis. Alternatively Lambda Mono Cut Mix can be separated by conventional gel electrophoresis using the following conditions: 0.5 μg Lambda Mono Cut Mix, 0.4 % agarose gel, 1X TAE, 10 V, 25°C for 24 hours.
- 1X Gel Loading Dye, Blue:
2.5% Ficoll-400
11 mMEDTA
3.3 mM Tris-HCL (pH 8.0@25°C)
0.017% SDS
0.015% bromophenol blue
FAQs
- How can I quantify the amount of DNA in each band of a marker?
- General information on Lambda DNA Mono Cut Mix:
Protocols
Protocols for λ DNA-Mono Cut Mix
References
- Daniels, D.L. et al. (1983) In Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg (Eds.), Lambda-II, pp. 519-676.
Reagents Sold Separately
Gel Loading Dye, Blue (6X)
Companion Products
1 kb DNA Ladder
100 bp DNA Ladder
2-Log DNA Ladder (0.1–10.0 kb)
PCR Marker
TriDye™ 1 kb DNA Ladder
TriDye™ 100 bp DNA Ladder
TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)
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