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λ DNA-BstEII Digest |
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Prices are in US dollars and valid only for US orders.
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Description:
The BstEII digest of lambda DNA (cI857 ind 1 Sam 7) yields 14 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
Lambda DNA-BstE II Digest visualized by ethidium bromide staining. 1.0% agarose gel.
Effective Size Range: 117 bp to 8,454 bp
Source:
The phage is isolated from the heat-inducible lysogen E. coli λ cI857 S7 and then isolated from the purified phage by phenol extraction and dialyzed.The double-stranded DNA is digested to completion with BstEII, phenol extracted and dialyzed against 10 mM Tris-HCl (pH 8.0) 1 mM EDTA.
Reagents Supplied:
Gel Loading Dye, Blue (6X)
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| 1 | | 8,454 | | 174 | | |
| 2 | | 7,242 | | 149 | | |
| 3 | | 6,369 | | 131 | | |
| 4 | | 5,686 | | 117 | | |
| 5 | | 4,822 | | 99 | | |
| 6 | | 4,324 | | 89 | | |
| 7 | | 3,675 | | 76 | | |
| 8 | | 2,323 | | 48 | | |
| 9 | | 1,929 | | 40 | | |
| 10 | | 1,371 | | 28 | | |
| 11 | | 1,264 | | 26 | | |
| 12 | | 702 | | 14 | | |
| 13 | | 224 | | 5 | | |
| 14 | | 117 | | 2 | | |
Concentration:
500 μg/ml
Recommended Load: 1 μg
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- 1X Gel Lodaing Dye, Blue:
2.5% Ficoll-400
11 mM EDTA
3.3 mM Tris-HCL (pH 8.0@25°C)
0.017% SDS
0.015% bromophenol blue
Usage notes:- For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20 and subsequently heated. Temperatures > 60°C may cause denaturation.
- The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.
FAQs
- What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
- How can I quantify the amount of DNA in each band of a marker?
- Why does my Lambda DNA molecular weight marker appear smeared and not represent the correct banding pattern?
References
- Daniels, D.L. et al (1983) In Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg (Eds.), Lambda-II, pp. 519-676.
- Forster, A.C. et al. (1985) Nucl. Acids Res., 13, 745-761.
Reagents Sold Separately
Gel Loading Dye, Blue (6X)
Companion Products
1 kb DNA Ladder
100 bp DNA Ladder
2-Log DNA Ladder (0.1–10.0 kb)
PCR Marker
TriDye™ 1 kb DNA Ladder
TriDye™ 100 bp DNA Ladder
TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)
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