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siRNA Marker |
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Prices are in US dollars and valid only for US orders.
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Description:
The siRNA Marker is a set of three annealed synthetic siRNA duplexes that are 17, 21 and 25 base pairs in length with 2-base 3´ overhangs. This marker is suitable for use as a siRNA size standard on native polyacrylamide gels (Figure 1).
Recommended Loading Amount: 5-10 µl per lane
Molecular Weight: The average molecular weight of the 21 siRNA duplex is ~ 13.300 g/mol.
Figure 1: 45 ng (5 µl) of siRNA Marker on a 20% TBE polyacrylamide gel electrophoresis.
Figure 2: Three synthetic annealed siRNA duplexes.
Concentration:
9 ng/μl
Storage Conditions
Storage Conditions:
1 mM EDTA
5% Glycerol
0.008% Orange G
Storage Temperature:
-20°C
Notes
Usage notes:- The duplexes share some sequence similarities (Figure 2). Therefore, incomplete denaturation/reannealing will result different size species. For denaturing gels, we recommend the microRNA Marker (NEB #N2102) be used.
Companion Products
microRNA Marker
ShortCut® RNAi Kit
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