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FAQs for DNA Modifying Enzymes and Cloning Technical Reference for DNA Modifying Enzymes and Cloning Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE NEBlot Kit

Home > Products > DNA Modifying Enzymes and Cloning > DNA Labeling and Chemiluminescent Detection > Octadeoxyribonucleotides in 10X Labeling Buffer Octadeoxyribonucleotides in 10X Labeling Buffer Catalog # Size Concentration Price Qty   N1501L 50 reactions 400 μg/ml $79.00 N1501S 25 reactions 400 μg/ml $42.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: This mixture of random octadeoxynucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). This primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labeled" by substituting any radiolabeled nucleotide for the appropriate non-radioactive nucleotide in the reaction mixture. Use of synthetic d(N)8 primer ensures the presence of virually all sequence combination of octamer primers which results in equally labeled DNA of high specific activity (1,2). Reaction & Storage Conditions Concentration: 400 μg/ml Storage Conditions: 0.5 M Tris-HCl 100 mM MgSO4 10 mM Dithiothreitol pH 7.2 @ 25°C Storage Temperature: -20°C Notes General notes: For use in the NEBlot Kit (NEB #N1500S) or standard random priming reactions. Usage notes: Add 5 µl of 10X Random Primer Solution per 50 µl reaction. References Feinberg, A.P. and Vogelstein, B. (1983) Anal. Biochem., 132, 6-13. Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem., 137, 266-267. Companion Products NEBlot Kit