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FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE DNA Gyrase (E. coli)

Home > Products > Nucleic Acids > Others > DNA Gyrase (E. coli) Substrate DNA Gyrase (E. coli) Substrate Catalog # Size Concentration Price Qty   N0471S 20 μg 1,000 μg/ml $61.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A double-stranded covalently closed circular DNA molecule produced by incubating supercoiled pUC19 DNA with Topoisomerase I (E. coli). Reaction & Storage Conditions Concentration: 1,000 μg/ml Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 8.0 @ -20°C Storage Temperature: -20°C Notes General notes: Preparation: pUC19 is isolated from E. coli ER2272 (dam+ dcm+ EcoK M– ) by a standard plasmid purification procedure. References Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Gene, 33, 103-119. Companion Products DNA Gyrase (E. coli)