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FAQs for Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb) FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE 1 kb DNA Ladder 100 bp DNA Ladder 2-Log DNA Ladder (0.1–10.0 kb) Quick-Load® 1 kb DNA Ladder Quick-Load® 100 bp DNA Ladder TriDye™ 1 kb DNA Ladder TriDye™ 100 bp DNA Ladder TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

Home > Products > Nucleic Acids > DNA Markers/Ladders > Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb) Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb) Catalog # Size Concentration Price Qty   N0469S 125 gel lanes 100 μg/ml $79.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Description: Quick-Load® 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands. Quick-Load 2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane. Effective Size Range: 100 bp to 10 kb Source: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Fragment Size (bp) Mass (ng) 1 10,002 40 2 8,001 40 3 6,001 48 4 5,001 40 5 4,001 32 6 3,001 120 7 2,017 40 8 1,517 57 9 1,200 45 10 1,000 122 11 900 34 12 800 31 13 700 27 14 600 23 15a 517 124 15b 500 124 16 400 49 17 300 37 18 200 32 19 100 61 Concentration: 100 μg/ml Recommended Load: 1 μg Storage Conditions Storage Conditions: 3.3 mM Tris-HCl 11 mM EDTA 0.015% Bromophenol Blue 0.017% SDS 2.5% Ficoll 400 pH 8.0 @ 25°C Storage Temperature: 4°C Notes General notes: Quick-Load 2-Log DNA Ladder is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. Usage notes: Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer. We recommend loading 10 μl (1.0 μg) or Quick-Load 2-Log DNA Ladder per gel lane. The Quick-Load 2-Log DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size. FAQs What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment? Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands? How can I quantify the amount of DNA in each band of a marker? References Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 10.51-10.67. Companion Products 1 kb DNA Ladder 100 bp DNA Ladder 2-Log DNA Ladder (0.1–10.0 kb) Quick-Load® 1 kb DNA Ladder Quick-Load® 100 bp DNA Ladder TriDye™ 1 kb DNA Ladder TriDye™ 100 bp DNA Ladder TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)