 | | | | Quick-Load® 1 kb DNA Ladder | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
Quick-Load® 1 kb DNA Ladder is a premixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.
The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases. The 3.0 kb fragment has increased intensity to serve as reference bands.
1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/lane.
Effective Size Range: 500 bp to 10 kb
Source:
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
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| 1 | | 10,002 | | 42 | | |
| 2 | | 8,001 | | 42 | | |
| 3 | | 6,001 | | 50 | | |
| 4 | | 5,001 | | 42 | | |
| 5 | | 4,001 | | 33 | | |
| 6 | | 3,001 | | 125 | | |
| 7 | | 2,000 | | 48 | | |
| 8 | | 1,500 | | 36 | | |
| 9 | | 1,000 | | 42 | | |
| 10a | | 517 | | 42 | | |
| 10b | | 500 | | 42 | | |
Concentration:
50 μg/ml
Recommended Load: 0.5 μg
Storage Conditions
Storage Conditions:
3.3 mM Tris-HCl
11 mM EDTA
0.015% Bromophenol Blue
0.017% SDS
2.5% Ficoll 400
pH 8.0 @ 25°C
Storage Temperature:
4°C
Notes
General notes:- Quick-Load 1 kb DNA Ladder is stable for at least 6 months at 25°C.
- For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.
Usage notes:- Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
- We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb DNA Ladder per gel lane.
- The Quick-Load 1 kb DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
FAQs
- Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
- How can I quantify the amount of DNA in each band of a marker?
References
- Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press
, 10.51?10.67.
Companion Products
1 kb DNA Ladder
100 bp DNA Ladder
2-Log DNA Ladder (0.1–10.0 kb)
50 bp DNA Ladder
Quick-Load® 100 bp DNA Ladder
Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb)
Quick-Load® 50 bp DNA Ladder
TriDye™ 1 kb DNA Ladder
TriDye™ 100 bp DNA Ladder
TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)
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