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FAQs for Quick-Load® 1 kb DNA Ladder FAQs for Markers and Ladders (DNA/RNA/Protein) Technical Reference for Markers and Ladders (DNA/RNA/Protein) Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE 1 kb DNA Ladder 100 bp DNA Ladder 2-Log DNA Ladder (0.1–10.0 kb) 50 bp DNA Ladder Quick-Load® 100 bp DNA Ladder Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb) Quick-Load® 50 bp DNA Ladder TriDye™ 1 kb DNA Ladder TriDye™ 100 bp DNA Ladder TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

Home > Products > Markers and Ladders (DNA/RNA/Protein) > DNA Markers/Ladders > Quick-Load® 1 kb DNA Ladder Quick-Load® 1 kb DNA Ladder Catalog # Size Concentration Price Qty   N0468L 375 gel lanes 50 μg/ml $140.00 N0468S 125 gel lanes 50 μg/ml $58.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Quick-Load® 1 kb DNA Ladder is a premixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases. The 3.0 kb fragment has increased intensity to serve as reference bands. 1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 µg/lane. Effective Size Range: 500 bp to 10 kb Source: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Fragment Size (bp) Mass (ng) 1 10,002 42 2 8,001 42 3 6,001 50 4 5,001 42 5 4,001 33 6 3,001 125 7 2,000 48 8 1,500 36 9 1,000 42 10a 517 42 10b 500 42 Concentration: 50 μg/ml Recommended Load: 0.5 μg Storage Conditions Storage Conditions: 3.3 mM Tris-HCl 11 mM EDTA 0.015% Bromophenol Blue 0.017% SDS 2.5% Ficoll 400 pH 8.0 @ 25°C Storage Temperature: 4°C Notes General notes: Quick-Load 1 kb DNA Ladder is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. Usage notes: Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer. We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb DNA Ladder per gel lane. The Quick-Load 1 kb DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size. FAQs Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands? How can I quantify the amount of DNA in each band of a marker? References Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press , 10.51?10.67. Companion Products 1 kb DNA Ladder 100 bp DNA Ladder 2-Log DNA Ladder (0.1–10.0 kb) 50 bp DNA Ladder Quick-Load® 100 bp DNA Ladder Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb) Quick-Load® 50 bp DNA Ladder TriDye™ 1 kb DNA Ladder TriDye™ 100 bp DNA Ladder TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb) New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201