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Home > Products > Polymerases and Amplification > Nucleotide Solutions > Ribonucleotide Solution Mix Ribonucleotide Solution Mix Catalog # Size Concentration Price Qty   N0466L 40 μmol of each 80 mM each nt $256.00 N0466S 8 μmol of each 80 mM each nt $64.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Ribonucleotide Solution Mix is a buffered equimolar solution of ribonucleotide triphosphates: rATP, rCTP, rGTP and rUTP. Each nucleotide is present at a concentration of 80 mM. (total rNTP concentration equals 320 mM). Reaction & Storage Conditions Concentration: 80 mM each nt Storage Conditions: 20 mM Tris-HCl 10 mM Dithiothreitol pH 7.8 @ 25°C Storage Temperature: -20°C Notes General notes: Storage: supplied as sodium salts in 20 mM Tris-HCl (pH7.8), 10 mM dithiothreitol. To ensure maximum activity upon long-term storage, aliquot and store at -80°C. Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. Quality Assurance Statement: Ribonucleotides are certified free of detectable ribonuclease activity. Functional purity is determined by preparative in vito transcription reactions. Functional Assay: A preparative HiScribe™ in vitro transcription reaction containing both Bgl II and Stu I-linearized LITMUS 28iMal templates, with rNTP's at a final concentration of 4 mM each, produced the expected 902 bp double-stranded RNA product at a yield of 0.89 pmol (0.52 µg) per microliter of reaction, as measured by α-[33]P ATP incorporation. Companion Products ShortCut® RNase III SP6 RNA Polymerase T7 RNA Polymerase New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201