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FAQs for DNA Modifying Enzymes and Cloning Technical Reference for DNA Modifying Enzymes and Cloning Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE Cre Recombinase

Home > Products > DNA Modifying Enzymes and Cloning > Recombinases > pLox2+ (linearized) pLox2+ (linearized) Catalog # Size Concentration Price Qty   N0417S 10 μg 125 ng/μl $53.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: pLox2+ (linearized) is a substrate for Cre Recombinase (NEB #M0298). It is 3.65 kb in length and has a loxP site approximately 350 bp from each end. Between the loxP sites lie an origin of replication and an ampicillin resistance gene. Recombination between these loxP sites by Cre Recombinase produces a circular, ampicillin-resistant plasmid (which migrates at approximately 1.7 kb on a 0.8% agarose gel) and a 850 bp DNA fragment. Source: pLox2+ is isolated from E. coli ER2502 by a standard purification procedure, digested to completion with XmnI, phenol/chloroform extracted, precipitated and resuspended in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA Concentration: 125 ng/μl Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C Storage Temperature: -20°C References Cantor, E. and Chong, S. (2001) Prot. Exp. Purif., 22, 135-140. Companion Products Cre Recombinase