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FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE HiScribe RNAi Transcription Kit ProtoScript® First Strand cDNA Synthesis Kit ShortCut® RNAi Kit siRNA Marker ssRNA Ladder T7 RNA Polymerase

Home > Products > Nucleic Acids > RNA Markers/Ladders > dsRNA Ladder dsRNA Ladder Catalog # Size Concentration Price Qty   N0363S 25 gel lanes 500 μg/ml $75.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Description: The dsRNA Ladder is a set of 7 annealed double-stranded RNA molecules produced by in vitro transcription of 14 linear DNA templates. The ladder sizes are: 500, 300, 150, 80, 50, 30 and 21 base pairs. All have 2-base, 3´ extensions. The 80 base pair fragment is at double intensity to serve as a reference point. This ladder is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels. Left: 1 µg of dsRNA Ladder was visualized by ethidium bromide staining on a 6% Polyacrylamide gel 10 V/cm. Right: 1.5 µg of dsRNA Ladder was visualized by ethidium bromide staining on a 2% TBE agarose gel 10 V/cm. Concentration: 500 μg/ml Storage Conditions Storage Conditions: 10 mM Tris-HCl 1 mM EDTA pH 8.0 @ 25°C Storage Temperature: -20°C Notes General notes: Double-stranded RNA is sensitive to degradation by ribonucleases, even though it is more resistant than single-stranded RNA. To avoid ribonuclease contamination: wear gloves, use RNase-free water for gels and buffers, wash equipment with detergent and rinse throughly with RNase-free water. Excessively long run times or high voltage can cause degradation of the bands on the gel. We recommend loading 1.0-1.5 µg of dsRNA Ladder, a running voltage of 4-10 V/cm , adding ethidium bromide to gels and running buffer at a final concentration of 0.5 µg/ml to effectively stain the bands during electrophoresis. Usage notes: This marker was not designed for precise quantification of dsRNA mass. References Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), (p. 7.43?7.45), Cold Spring Harbor Laboratory Press. Liu, Y-C. and Chou, Y-C. (1990) Biotechniques, 9, 558. Dong Ma, New England BioLabs, Inc., unpublished observations. Companion Products HiScribe RNAi Transcription Kit ProtoScript® First Strand cDNA Synthesis Kit ShortCut® RNAi Kit siRNA Marker ssRNA Ladder T7 RNA Polymerase