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Protocols for ssRNA Ladder FAQs for Nucleic Acids Technical Reference for Nucleic Acids Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE ssRNA Ladder Loading Buffer dsRNA Ladder HiScribe RNAi Transcription Kit ProtoScript® First Strand cDNA Synthesis Kit siRNA Marker T7 RNA Polymerase

Home > Products > Nucleic Acids > RNA Markers/Ladders > ssRNA Ladder ssRNA Ladder Catalog # Size Concentration Price Qty   N0362S 25 gel lanes 500 μg/ml $58.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Description: The ssRNA Ladder is a set of 7 RNA molecules produced by in vitro transcription of a mixture of 7 linear DNA templates. The ladder sizes are: 9000, 7000, 5000, 3000, 2000, 1000 and 500 bases. The 3000 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an RNA size standard on denaturing or native agarose gels. 1 µg of ssRNA Ladder was heated at 60°C for 5 minutes in 1X ssRNA Ladder Sample Buffer and visualized by ethidium bromide staining (1.0% TBE agarose gel). Effective Size Range: 500 bp to 9,000 bp Reagents Supplied: ssRNA Ladder Loading Buffer (2X) Concentration: 500 μg/ml Recommended Load: 1 μg Storage Conditions Storage Conditions: 20 mM potassium acetate pH 4.5 @ 25°C Storage Temperature: -70°C Notes General notes: To avoid ribonuclease contamination: wear gloves, use RNase-free water for gels and buffers, wash equipment with detergent and rinse thoroughly with RNase-free water. It is best to use freshly poured gels that are as thin as possible (i.e. 2-10 mm). Excessively long run times or high voltage can cause degradation of the bands on the gel. We recommend 4-8 volts/cm and running the bromphenol blue approximately 5 cm into the gel for good resolution. Adding ethidium bromide to agarose gels and running buffer at a final concentration of 0.5 µg/ml will effectively stain the bands during electrophoresis. For short term storage (< 1 Week), ladder can be stored at -20°C. This marker was not designed for precise quantification of ssRNA mass. Usage notes: Denaturing vs. Native Agarose Gels: It is common practice to electrophorese RNA on a fully denaturing agarose gel, such as one containing formaldehyde (1). However, in many cases it is possible to run RNA on a native agarose gel and obtain suitable results. In fact, it has been demonstrated that treatment of RNA samples in a denaturing buffer maintains the RNA molecules in a denatured state, during electrophoresis, for at least 3 hours (2,3). The use of native agarose gels eliminates problems associated with toxic chemicals and the difficulties encountered when staining and blotting formaldehyde gels. References Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd ed.), 7.43-7.45. Liu, Y.-C. and Chou, Y.-C. (1990) Biotechniques, 9, 558. Cook, S. and Marchetti, C., unpublished observations. Reagents Sold Separately ssRNA Ladder Loading Buffer Companion Products dsRNA Ladder HiScribe RNAi Transcription Kit ProtoScript® First Strand cDNA Synthesis Kit siRNA Marker T7 RNA Polymerase