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RELATED INFORMATION FAQs for Yeast Chromosome PFG Marker FAQs for Markers and Ladders (DNA/RNA/Protein) Technical Reference for Markers and Ladders (DNA/RNA/Protein) FAVORITE TOOLS Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE RELATED PRODUCTS Companion Products Lambda Ladder PFG Marker Low Range PFG Marker MidRange I PFG Marker MidRange II PFG Marker

Home > Products > Markers and Ladders (DNA/RNA/Protein) > PFG Markers > Yeast Chromosome PFG Marker Yeast Chromosome PFG Marker Catalog # Size Concentration Price Qty   N0345S 50 gel lanes 50 μg/ml $61.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Chromosomes isolated from Saccharomyces cerevisiae, strain YPH80 embedded in 1% LMP agarose and supplied in a GelSyringe™ dispenser. Designed for use as size markers for pulsed-field gel electrophoresis (PFG). Size range: 225-1,900 kb. 1% agarose gel, 6.0 V/cm, 15°C for 26 hours. Switch time of 70 seconds for 15 hours and 120 seconds for 11 hours. Effective Size Range: 225 kb to 1,900 kb Source: Saccharomyces cerevisiae YPH80 Chromosome Size (kb) XII 1,900 IV 1,640 VII 1,120 XV 1,100 XVI 945 XIII 915 II 815 XIV 785 X 745 XI 680 V 610 VIII 555 IX 450 III 375 VI 295 I 225 Concentration: 50 μg/ml Storage Conditions Storage Conditions: 0.25 M EDTA 50% Glycerol 1% LMP agarose pH 9.0 @ 25°C Storage Temperature: -20°C Notes General notes: The photograph represents the pulsed field gel separation of Yeast Chromosomes using a CHEF apparatus. The 1% agarose gel was run at 6 volts/cm using pulse times of 70 seconds (15 hours) and 120 seconds (11 hours) at 15°C in 0.5X TBE buffer (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water, allowing resolution of 15 of the 16 Yeast Chromosomes. Chromosomes VII and XV appear as a single band. Chromosome XII may display anomalous electrophoretic behavior. Usage notes: Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose. Allow no bubbles to form. Recommended plug size is 20 µl (two small graduations on the syringe volume scale), which contains approximately 1 µg of DNA. Each syringe yields 25+ plugs. FAQs There are several factors that lead to poor results for PFG markers. Here is a list of recommendations for ensuring a sharp high quality banding pattern. References Carle, G. and Olson, M. (1985) Proc. Natl. Acad. Sci. USA, 82, 3756. Mortimer, R. and Schild, D. (1985) Microbiol. Rev., 49, 181. Claus, T. and Akbari, T., unpublished observations. Companion Products Lambda Ladder PFG Marker Low Range PFG Marker MidRange I PFG Marker MidRange II PFG Marker