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Markers and Ladders (DNA/RNA/Protein) >
PFG Markers >
Lambda Ladder PFG Marker |
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Prices are in US dollars and valid only for US orders.
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Description:
Successively larger concatemers of lambda DNA (cI857 ind 1 Sam7) embedded in 1% LMP agarose and supplied in a GelSyringe™ dispenser. Designed to be used as size markers for pulsed-field gel electrophoresis (PFG). Size range: 50-1,000 kb.
1% agarose gel, 4.5 V/cm, 15°C for 48 hours. Switch times ramped from
5-120 seconds.
Effective Size Range: 48.5 kb to 1,018.5 kb
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| 21 | | 1,018.5 | | |
| 20 | | 970.0 | | |
| 19 | | 921.5 | | |
| 18 | | 873.0 | | |
| 17 | | 824.5 | | |
| 16 | | 776.0 | | |
| 15 | | 727.5 | | |
| 14 | | 679.0 | | |
| 13 | | 630.5 | | |
| 12 | | 582.0 | | |
| 11 | | 533.5 | | |
| 10 | | 485.0 | | |
| 9 | | 436.5 | | |
| 8 | | 388.0 | | |
| 7 | | 339.5 | | |
| 6 | | 291.0 | | |
| 5 | | 242.5 | | |
| 4 | | 194.0 | | |
| 3 | | 145.5 | | |
| 2 | | 97.0 | | |
| 1 | | 48.5 | | |
Concentration:
50 μg/ml
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
50% Glycerol
1% LMP agarose
pH 8.0 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- The photograph represents the pulsed field gel separation of Lambda Ladders using a CHEF apparatus. The 1% agarose gel was run at 4.5 volts/cm using ramped pulse times from 5 to 120 seconds for 48 hours at 15°C in 0.5X TBE (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water allowing resolution of 20 Lambda Ladder bands.
Usage notes:- Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose. Allow no bubbles to form.
- Recommended plug sizes are from 5-10 µl. A 10 µl plug (one small graduation on the GelSyringe volume scale) contains approximately 0.5 µg of DNA. Each GelSyringe yields 25+ plugs.
- Melting plugs will cause denaturation of concatemers.
FAQs
- There are several factors that lead to poor results for PFG markers. Here is a list of recommendations for ensuring a sharp high quality banding pattern.
References
- Ellard, J., Greci, J. and Davis, T.B., unpublished observations.
- Burke-Aguaro, D., unpublished observations.
Companion Products
Low Range PFG Marker
MidRange I PFG Marker
MidRange II PFG Marker
Yeast Chromosome PFG Marker
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