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TransPass™ P Protein Transfection Reagent |
 | | | | TransPass™ P Protein Transfection Reagent | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
This product includes 0.125 ml of TransPass™ P and 0.02 ml of Control Protein.
TransPass P is a transfection reagent that can deliver proteins and peptides at high transfection efficiency into live mammalian cells via endocytosis. The activity of delivered proteins is maintained so active enzymes and other bio-molecules such as antibodies can be directly delivered to cells bypassing the need of obtaining and transfecting a DNA expression construct.
TransPass P Transfection Reagent forms a non-covalent association with the protein which protects the protein from degradation upon endocytosis. It allows the study of protein activity in living cells without the need of permeabilization and fixing.
TransPass P Transfection Reagent has a broad range of cell type specificity, minimal toxicity, and an efficiency of transfection higher than typical plasmid DNA transfection. A fluorescently labeled control protein (IgG labeled with DyLight 549) is included for optimization of transfection conditions.
Cell Lines Successfully Transfected: HUVEC (Human Umbilical Vein Endothelial Cells), NIH3T3, MCF-7, COS-7, HepG2, HeLa, CHO-K1 and Huh-7 cells.
Figure 1: NIH3T3 cells transfected for 2 hours with 6 µl TransPass P Protein Transfection Reagent and 1 µg IgG labeled with a fluorescent dye (DyLight 549). The cells were fixed, DAPI stained and photographed 24 hours post-transfection.
Figure 2: HUVEC cells transfected with an antibody labeled with Oregon Green and TransPass P Transfection Reagent. 1µg of antibody was delivered with 2 µl of TransPass P. The cells were fixed and photographed 24 hours post- transfection.
Figure 3: NIH-3T3 cells were transfected with 1 µg of a fluorescent peptide (Flu-P1 NEB#P6606) and the indicated amounts of TransPass P Transfection Reagent in a 6-well plate format. The cells were photographed 24 hours post-transfection.
Reaction & Storage Conditions
Storage Temperature:
4°C
Notes
General notes:- Storage: TransPass P Transfection Reagent should be stored at 4°C. The fluorescent-labeled control protein (IgG labeled with DyLight 549) should be protected from light and stored at 4°C.
- TransPass P is a proprietary formulation manufactured by Targeting Systems. Please direct any inquiries regarding reagent composition to Targeting Systems.
Usage notes:- Use purified proteins and enzymes free of bacteria or toxins.
- The amount of protein and the incubation time with the transfection complex mixture used may be adjusted for optimal transfection efficiency.
- Use 0.5-1 µl of the control protein per transfection (6-well plate format).
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
Each lot of transfection reagent is tested for efficient delivery of the control protein.
Companion Products
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Rhodamine-conjugated Chitin-binding Probe
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