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DNA Modifying Enzymes and Cloning >
Phosphatases and Sulfurylases >
ATP Sulfurylase |
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- Isolated from a recombinant source
Description:
ATP Sulfurylase catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi.
Source:
An E.coli strain carrying a plasmid expressing the S. cerevisiae gene MET3.
Reaction & Storage Conditions
Unit Definition:
One unit is defined as the amount of enzyme that catalyzes the conversion of 1 µmol of APS and PPi to ATP in one minute at 30°C in a total reaction volume of 40 µl.
Unit Assay Conditions:
115 mM Tris-HCl (pH 8.0), 0.58 mM b-NADP, 2.4 mM Mg acetate, 34 mM D-glucose, 0.3 mM adenosine 5´-phosphosulfate, 3.4 mM pyrophosphate, 0.75 units/ml hexokinase and 0.5 units/ml glucose 6-phosphate dehydrogenase.
Concentration:
300 units/ml
Storage Conditions:
10 mM Tris-HCl
50 mM NaCl
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
Storage Temperature:
-20°C
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Phosphatase Contamination:
After incubation of 1.5 units of ATP Sulfurylase with 0.05 µmol p-nitrophenol phosphate for 20 hours at 37°C, no phosphatase activity could be detected by spectrophotometric analysis.
Nuclease Contamination:
Incubation of 1.5 units of ATP Sulfurylase for 20 hours in the recommended assay buffer with 2-log DNA Ladder revealed no detectable endonuclease activity as determined by agarose gel electrophoresis.
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