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Crimson Taq™ DNA Polymerase with (Mg-free) Buffer |
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Prices are in US dollars and valid only for US orders.
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Description:
Crimson Taq™ DNA Polymerase with (Mg-free) Buffer offers superior performance in its newly formulated PCR buffer. Crimson Taq (Mg-free) Reaction Buffer contains a density reagent, which allows direct loading of PCR products onto a gel. In addition, Crimson Taq (Mg-free) Buffer has trace amounts of a red dye, which serves as an inidicator for homogenous reaction set-up, a color aid in gel loading and a tracking dye which migrates at about 10 bp on a 1% TBE gel.
Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Applications:- Primer Extension
- Routine PCR
- Colony PCR
- DHPLC
- Microarray Analysis
- High-throughput PCR
Advantages:- Robust and reliable reactions
- Samples can be loaded directly onto a gel
- Ideal for high throughput applications
Reagents Supplied:
Crimson Taq™ (Mg-free) Reaction Buffer Pack
MgCl2 (10X)
Enzyme Properties
Heat Inactivation:
No
Reaction & Storage Conditions
Unit Definition:
One unit of Crimson Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Unit Assay Conditions: 1X ThermoPol Reaction Buffer, 200 μM dNTPs including [3H]-dTTP and 200 μg/ml activated Calf Thymus DNA.
Reaction Conditions:
1X Crimson™ Taq (Mg-free) Reaction Buffer
Supplemented with 200 μM dNTPs, 1.5 mM MgCl2 and 1.25-2.5 units of Taq DNA Polymerase.
1X Crimson Taq (Mg-free) Reaction Buffer:
12.5 mM Tricine (pH 8.5 @ 25°C)
42.5 mM KCl
6% Dextran
Acid Red
Concentration:
5,000 units/ml
Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% NP-40
pH 7.4 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- Store at -20°C for two years.
FAQs
- What are the advantages or disadvantages of Crimson Taq DNA Polymerase with (Mg-free) Buffer?
- Does the 5X Crimson Taq (Mg-free) Reaction Buffer offer amplification efficiency similar to that of Standard Taq Reaction Buffer or ThermoPol Reaction Buffer?
- Can the PCR product be used directly in T/A cloning?
- How do I remove the dye and Dextran from my PCR reactions using Crimson Taq (Mg-free)Reaction Buffer?
- How long is the Crimson Taq Reaction buffer stable in non-optimal storage conditions?
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
5 kb Lambda PCR:
25 cycles of PCR amplification of 5 ng Lambda DNA with 2.5 units of Crimson Taq DNA Polymerase in the presence of 200 μM dNTPs, 0.2 μM primers, 1.5 mM MgCl2 and 1X Crimson Taq (Mg-free) Reaction Buffer resulted in a specific product.
Endonuclease Activity:
Incubation of 20 units of Taq DNA Polymerase with ThermoPol Reaction Buffer with 1 μg ϕX174 RF I DNA for 4 hours at 75°C in a 50 μl reaction resulted in <10% conversion to RF II as determined by agarose gel electrophoresis.
References
- Barnes, W.M. (1994) Proc. Natl. Acad. Sci., 91, 2216-2220.
- Foord, O.S. and Rose, E.A. (1994) PCR Methods Appl., 3, 149-161.
- Sun, Y. et al. (1993) Biotechniques, 15, 372-374.
- Sarkar, G. et al. (1990) Nucleic Acids Res, 18, 7465.
Reagents Sold Separately
Crimson Taq™ (Mg-free) Reaction Buffer Pack
Companion Products
Crimson Taq™ DNA Polymerase
Crimson Taq™ Reaction Buffer Pack
Legal
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending on the particular application in which the product is used.
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