 | | | | LongAmp™ Taq DNA Polymerase | | | |
|
|
|
 |
|
Prices are in US dollars and valid only for US orders.
|
Description:
LongAmp™ Taq DNA Polymerase is a unique blend of Taq and Deep VentR® DNA Polymerases. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq Polymerase (1). LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq DNA Polymerase alone. A wide range of PCR products can be generated, up to 40 kb from lambda DNA and 30 kb from human genomic DNA.
Amplification of specific sequences from human genomic DNA using LongAmp Taq DNA Polymerase. Amplicon sizes are indicated below the gel. Marker M is the NEB 1 kb DNA Ladder (NEB #N3232).
Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.
Applications:Reagents Supplied:
LongAmp™ Taq Reaction Buffer Pack (5X)
Enzyme Properties
Heat Inactivation:
No
Reaction & Storage Conditions
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
Unit Assay Conditions: 1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Reaction Conditions:
1X LongAmp Taq Reaction Buffer, DNA template, primers, 300 µM dNTPs (not included) and 5 units of LongAmp Taq DNA Polymerase in a total reaction volume of 50 µl.
1X LongAmp™ Taq Reaction Buffer:
60 mM Tris-SO4
20 mM (NH4)2SO4
3 % Glycerol
0.06 % NP-40
0.05 % Tween-20
2 mM MgSO4
pH 9.0 @ 25°C
Concentration:
2,500 units/ml
Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% NP-40
pH 7.4 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- Store at -20°C for two years. We recommend -80°C for long term storage.
FAQs
- What is the recommended enzyme amount when using LongAmp?
- What is the fidelity of the LongAmp Taq DNA Polymerase compared to Taq DNA Polymerase?
- Can the extension step be carried out at 72°C when using LongAmp?
- What is the extension rate when using LongAmp?
- What type of DNA ends result from a primer extension reaction or a PCR reaction using LongAmp Taq DNA Polymerase?
- Why is the product a smear when visualized on an agarose gel?
- Can LongAmp Taq DNA Polymerase be used to amplify GC-rich amplicons?
Protocols
Protocols for LongAmp™ Taq DNA Polymerase
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Long Amplicon PCR:
LongAmp Taq DNA Polymerase is tested for the ability to amplify a 40 kb amplicon from lambda DNA and a 30 kb amplicon from human genomic DNA.
References
- Barnes, W.M. (1994) Proc. Natl. Acad. Sci., 91, 2216-2220.
- Saiki R.K. et al. (1985) Science, 230, 1350-1354.
- Powell, L.M. et al. (1987) Cell, 50, 831-840.
- Sun, Y., Hegamyer, G. and Colburn, N. (1993) Biotechniques, 15, 372-374.
- Sarkar, G., Kapelner, S. and Sommer, S.S. (1990) Nucleic Acids Res., 18, 7465.
Reagents Sold Separately
LongAmp™ Taq Reaction Buffer Pack
Companion Products
Crimson LongAmp™ Taq DNA Polymerase
Deoxynucleotide Solution Mix
Deoxynucleotide Solution Set
Diluent F
LongAmp™ Taq (Mg-free) Reaction Buffer Pack
LongAmp™ Taq 2X Master Mix
LongAmp™ Taq PCR Kit
LongAmp™ Taq Reaction Buffer Pack
Magnesium Sulfate (MgSO4) Solution
Legal
Patents:
New England Biolabs, Inc.: 5,352,778; 5,500,363 and 5,834,285
Licenses/Patents/Disclaimers:
The purchase of this product conveys to the purchaser only the limited, non-transferable right to use the purchased quantity of the product for the purchaser's own research by the purchaser only under the following U.S. patent claims and foreign counterpart patent claims: U.S. Patent Number 5,436,149 (claims 6-16). No rights are granted to the purchaser to sell, modify for resale or otherwise transfer this product, either alone or as a component of another product, to any third party. Takara Bio reserves all other rights, and this product may not be used in any manner other than as provided herein. For information on obtaining a license to use this product for purposes other than research, please contact Takara Bio Inc., Seta 3-4-1, Otsu, Shiga 520-2193, Japan (Fax +81-77-453-9254).
|
