 | | | | Thermostable Inorganic Pyrophosphatase | | | |
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- Retains 100% activity after incubation at 100°C for 4 hours
Description:
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
P2O7-4 + H2O -> 2HPO4-2.
Source:
A E. coli strain carrying a plasmid encoding pyrophosphatase from the extreme thermophile Thermococcus litoralis.
Enzyme Properties
Heat Inactivation:
No
Reaction & Storage Conditions
Unit Definition:
One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Concentration:
2,000 units/ml
Storage Conditions:
20 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C
Storage Temperature:
-20°C
References
- Heinonen, J.K. and Lahti, R.J. (1981) Analytical Biochemistry, 113, 313-317.
Legal
Patents:
New England Biolabs, Inc.: U.S. Patent No. 5,861,296
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