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LongAmp™ Taq 2X Master Mix |
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Prices are in US dollars and valid only for US orders.
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Description:
The LongAmp™ Taq 2X Master Mix combines high quality NEB recombinant Taq DNA Polymerase with a trace amount of Deep VentR® DNA Polymerase. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). This ready-to-use mix offers up to six fold higher fidelity than Taq DNA Polymerase alone. A wide range of PCR products can be generated, up to 40 kb from lambda DNA and 30 kb from human genomic DNA (Figure 1).
Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.
Applications:Reagents Supplied:
LongAmp™ Taq Master Mix
Enzyme Properties
Heat Inactivation:
No
Reaction & Storage Conditions
Reaction Conditions:
1X LongAmp™ Taq Master Mix
Incubate at
65°C.
1X LongAmp™ Taq Master Mix:
60 mM Tris-SO4
2 mM MgSO4
0.3 mM dNTPs
5 % Glycerol
0.06 % NP-40
0.05 % Tween-20
125 units/ml LongAmp Taq DNA Polymerase
20 mM ammonium sulfate
pH 9.0 @ 25°C
Unit Definition:
One unit of LongAmp Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C.
Unit Assay Conditions: 1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Concentration:
2 X
Storage Temperature:
-20°C
Notes
General notes:- Store at -20°C for one year. We recommend -80°C for long term storage. For daily use, keep an aliquot at 4°C up to three months. The master mix is stable for at least 15 freeze-thaw cycles.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Long Amplicon PCR:
The master mix is tested for the ability to amplify a 40 kb amplicon from lambda DNA and a 30 kb fragment from human genomic DNA.
References
- Barnes, W.M. (1994) Proc. Natl. Acad. Sci., 91, 2216-2220.
- Foord, O.S. and Rose, E.A. (1994) PCR Methods Appl., 3, 149-161.
- Sun, Y. et al. (1993) Biotechniques, 15, 372-374.
- Sarkar, G. et al. (1990) Nucleic Acids Res., 18, 7465.
Companion Products
LongAmp™ Taq (Mg-free) Reaction Buffer Pack
LongAmp™ Taq DNA Polymerase
LongAmp™ Taq PCR Kit
LongAmp™ Taq Reaction Buffer Pack
Legal
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
The purchase of this product conveys to the purchaser only the limited, non-transferable right to use the purchased quantity of the product for the purchaser's own research by the purchaser only under the following U.S. patent claims and foreign counterpart patent claims: U.S. Patent Number 5,436,149 (claims 6-16). No rights are granted to the purchaser to sell, modify for resale or otherwise transfer this product, either alone or as a component of another product, to any third party. Takara Bio reserves all other rights, and this product may not be used in any manner other than as provided herein. For information on obtaining a license to use this product for purposes other than research, please contact Takara Bio Inc., Seta 3-4-1, Otsu, Shiga 520-2193, Japan (Fax +81-77-453-9254).
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