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Uracil Glycosylase Inhibitor (UGI) |
 | | | | Uracil Glycosylase Inhibitor (UGI) | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
The Uracil Glycosylase Inhibitor (UGI) of Bacillus subtilis bacteriophage PBS1 is a small protein (9.5 kDa) which inhibits E. coli uracil-DNA glycosylase (UDG) as well as UDG from other species. Inhibition of UDG occurs by reversible protein binding with a 1:1 UGD:UGI stoichiometry. UGI is capable of dissociating UDG-DNA complexes.
Source:
An E. coli strain that carries the cloned UGI gene from Bacillus subtilis bacteriophage PBS1.
Applications:- Uracil glycosylase inhibitor (UGI) inhibits uracil-DNA glycosylase (UDG). Since UDG remains partially active following heat treatment at 95°C, UGI can be used to prevent subsequent degradation of product DNA.
Reagents Supplied:
UDG Reaction Buffer
Enzyme Properties
Heat Inactivation:
No
Reaction & Storage Conditions
Reaction Conditions:
1X UDG Reaction Buffer
Incubate at
37°C.
1X UDG Reaction Buffer:
20 mM Tris-HCl
1 mM Dithiothreitol
1 mM EDTA
pH 8.0 @ 25°C
Unit Definition:
One unit of UGI is defined as the amount of protein required to inhibit one unit of E. coli UDG in 1 hour at 37°C in a total reaction volume of 50 µl. One unit of UDG is the amount of enzyme which will catalyze the release of 60 pmol of uracil per minute from double stranded, uracil-containing DNA.
Concentration:
1,000 units/ml
Storage Conditions:
20 mM Tris-HCl
50 mM NaCl
1 mM EDTA
0.1 mg/ml BSA
50% Glycerol
pH 8.0 @ 37°C
Storage Temperature:
-20°C
References
- Lindahl, T. et al. (1977) J. Biol. Chem., 252, 3286-3294.
- Wang, Z., et al. (1991) Gene, 99, 31-37.
- Bennett, S. E. and Mosbaugh, D. W. (1992) J. Biol. Chem., 267, 22512-22521.
Reagents Sold Separately
UDG Reaction Buffer
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