Incorporates dUTP, dITP and fluorescently-labeled nucleotides
Exceptional value in terms of cost per unit
Description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity.
It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
Applications:
PCR
Primer Extension
DHPLC
Microarray Analysis
High-Throughput PCR
Reagents Supplied: Standard Taq Reaction Buffer Pack
Enzyme Properties Heat Inactivation: No
Reaction & Storage Conditions Reaction Conditions: 1X Standard Taq Reaction Buffer 1X Standard Taq Reaction Buffer: 10 mM Tris-HCl 50 mM KCl 1.5 mM MgCl2
pH 8.3 @ 25°C
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Unit Assay Conditions: 1X Standard Taq Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Concentration: 5,000 units/ml
Storage Conditions: 10 mM Tris-HCl 100 mM KCl 1 mM Dithiothreitol 0.1 mM EDTA 50% Glycerol 0.5% Tween-20 0.5% NP-40
pH 7.4 @ 25°C
Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product.
Endonuclease Activity:
Incubation of a 50 μl reaction containing 20 units of Taq DNA Polymerase with Standard Taq Buffer with 1 μg of
ΦX174 RF I DNA for 4 hours at 75ºC resulted
in < 10% conversion to RFII as determined by agarose gel electrophoresis.
5 kb Lambda PCR: 25 cycles of PCR amplification of 5 ng Lambda DNA with 2.5 units of Taq DNA Polymerase in the presence of 200 µM dNTPS, 0.2 µM primers and 1X Standard Taq Reaction Buffer resulted in a yield of 100 ng specific product.
3´→ 5´ Exonuclease Activity: Incubation of 20 units of enzyme in 20 µl of a 10 nM solution of a fluorescent 5´– FAM labeled oligonucleotide for 30 minutes at 37°C and 75°C yields no detectable 3´→ 5´ degradation as determined by high resolution denaturing PAGE.
Legal Licenses/Patents/Disclaimers: Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
