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RELATED INFORMATION FAQs for Taq DNA Polymerase with Standard Taq Buffer Protocols for Taq DNA Polymerase with Standard Taq Buffer FAQs for Polymerases and Amplification Technical Reference for Polymerases and Amplification FAVORITE TOOLS Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE RELATED PRODUCTS Reagents Sold Separately Standard Taq Reaction Buffer Pack Companion Products Taq 5X Master Mix Taq 2X Master Mix Taq PCR Kit Crimson™ Taq PCR Sampler Deoxynucleotide Solution Mix Deoxynucleotide Solution Set Diluent F Magnesium Chloride (MgCl2) Solution Quick-Load® Taq 2X Master Mix Standard Taq (Mg-free) Reaction Buffer Pack

Home > Products > Polymerases and Amplification > Routine PCR > Taq DNA Polymerase with Standard Taq Buffer Taq DNA Polymerase with Standard Taq Buffer Catalog # Size Concentration Price Qty   M0273L 2,000 units 5,000 units/ml $232.00 M0273S 400 units 5,000 units/ml $58.00 M0273X 4,000 units 5,000 units/ml $418.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Isolated from a recombinant source Supplied with 10X Reaction Buffer Robust and reliable reactions Tolerates a wide range of templates Incorporates dUTP, dITP and fluorescently-labeled nucleotides Exceptional value in terms of cost per unit Description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems. Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 Applications: PCR Primer Extension DHPLC Microarray Analysis High-Throughput PCR Reagents Supplied: Standard Taq Reaction Buffer Pack (10X) Enzyme Properties Heat Inactivation: No Reaction & Storage Conditions Unit Definition: One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. Unit Assay Conditions: 1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.  Reaction Conditions:  1X Standard Taq Reaction Buffer, DNA template, primers, 200 µM dNTPs (not included) and 1.25 units of Taq DNA Polymerase in a total reaction volume of 50 µl.  1X Standard Taq Reaction Buffer: 10 mM Tris-HCl 50 mM KCl 1.5 mM MgCl2 pH 8.3 @ 25°C Concentration: 5,000 units/ml Storage Conditions: 10 mM Tris-HCl 100 mM KCl 1 mM Dithiothreitol 0.1 mM EDTA 50% Glycerol 0.5% Tween-20 0.5% NP-40 pH 7.4 @ 25°C Storage Temperature: -20°C FAQs Can Taq DNA Polymerase be used in other buffers? How should I set up a PCR reaction using Taq DNA Polymerase? Why is the product a smear when visualized on an agarose gel? Why is there no product when visualized on an agarose gel? What type of DNA ends result from a primer extension reaction or a PCR reaction using LongAmp Taq DNA Polymerase? The product sequence doesn't completely match the expected sequence. How can this result be improved? When should Taq DNA Polymerase be used in a primer extension reaction or for PCR? Does the presence of Ca2+ inhibit PCR reactions? Will the 5'→3' exonuclease activity of Taq DNA Polymerase degrade primers? Can Taq DNA Polymerase be used for nick translation? Protocols Protocols for Taq DNA Polymerase with Standard Taq Buffer Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. 5 kb Lambda PCR: 25 cycles of PCR amplification of 5 ng Lambda DNA with 1.25 units of Taq DNA Polymerase in the presence of 200 µM dNTPs and 0.2 µM primers in Standard Taq Reaction Buffer results in the expected 5 kb product. 3´→ 5´ Exonuclease Activity: Incubation of a 20 µl reaction in ThermoPol Reaction Buffer containing a minimum of 20 units of Taq DNA Polymerase with 10 nM fluorescent internally labeled oligonucleotide for 30 minutes at either 37°C or 75°C yields no detectable 3´→ 5´ degradation as determined by capillary electrophoresis. Endonuclease Assay: Incubation of a 50 µl reaction containing 20 units of Taq DNA Polymerasewith 1 µg of supercolied φX174 DNA for 4 hours at 75ºC resulted in < 10% conversion to the nicked form as determined by agarose gel electrophoresis. References Chien, A., Edgar, D.B. and Trela, J.M. (1976) J. Bact., 127, 1550-1557. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya, 45, 644-651. Lawyer, F.C. et al.  (1993) PCR Methods and Appl., 2, 275-287. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990) Nucleic Acids Res., 18, 7317-7322. Lyamichev, V., Brow, M.A. and Dahlberg, J.E.  (1993) Science, 260, 778-783. Saiki R.K. et al. (1985) Science, 230, 1350-1354. Powell, L.M. et al. (1987) Cell, 50, 831-840. Sun, Y., Hegamyer, G. and Colburn, N. (1993) Biotechniques, 15, 372-374. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990) Nucleic Acids Res., 18, 7465. Reagents Sold Separately Standard Taq Reaction Buffer Pack Companion Products Taq 5X Master Mix Taq 2X Master Mix Taq PCR Kit Crimson™ Taq PCR Sampler Deoxynucleotide Solution Mix Deoxynucleotide Solution Set Diluent F Magnesium Chloride (MgCl2) Solution Quick-Load® Taq 2X Master Mix Standard Taq (Mg-free) Reaction Buffer Pack