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Quick-Load® Taq 2X Master Mix |
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Prices are in US dollars and valid only for US orders.
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- Robust and reliable reactions
- Tolerates a wide range of templates
- Incorporates dUTP, dITP and fluorescently-labeled nucleotides
- Exceptional value in terms of cost per unit
Description:
Quick-Load® Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl, tracking dyes and stabilizers. The presence of two commonly used tracking dyes for DNA gels, Orange G and Xylene Cyanol FF, gives the master mix a green color, allowing direct loading of the PCR reaction product onto agarose gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates approximately 4 kb and Orange G migrates approximately 50 bp. The amount of tracking dyes included does not mask co-migrating DNA bands. Quick-Load Taq 2X Master Mix is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
Taq DNA Polymerase is a thermostable DNA polymerase that posesses a 5´→ 3´ polymerase activity and a double-strand specific 5´→ 3´ exonuclease activity.
Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Applications:- PCR
- Primer Extension
- Microarray Analysis
- High-Throughput PCR
Reagents Supplied:
MgCl2 (25 mM)
Quick-Load Taq MasterMix
Enzyme Properties
Heat Inactivation:
No
Reaction & Storage Conditions
Reaction Conditions:
1X Quick-Load Taq MasterMix
Supplemented with 25 mM MgCl2
Incubate at
75°C.
1X Quick-Load Taq MasterMix:
10 mM Tris-HCl
0.002 % Xylene Cyanol FF
50 mM KCl
1.5 mM MgCl2
0.2 mM dNTPs
5 % Glycerol
0.024 % Orange G
0.08 % NP-40
0.05 % Tween-20
50 units/ml Taq DNA Polymerase
pH 8.6 @ 25°C
Unit Definition:
One unit of Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Unit Assay Conditions:
1X Standard Taq Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Concentration:
2 X
Storage Temperature:
-20°C
Notes
General notes:- Keep at -20°C for long term storage. Quick-Load Taq 2X Master Mix is stable after repetitive freeze-thaw cycles. After three month storage at 4°C, the Quick-Load Taq 2X Master Mix gives about 50% yield on a 5kb amplicon using lambda DNA as template.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
5 kb Lambda PCR:
25 cycles of PCR amplification of 5 ng Lambda DNA with 2.5 units of Taq DNA Polymerase in the presence of 200 µM dNTPS, 0.2 µM primers and 1X Standard Taq Reaction Buffer resulted in a yield of 100 ng specific product.
3´→ 5´ Exonuclease Activity:
Incubation of 20 units of enzyme in 20 µl of a 10 nM solution of a fluorescent 5´- FAM labeled oligonucleotide for 30 minutes at 37°C and 75°C yields no detectable 3´→ 5´ degradation.
Endonuclease Activity:
Incubation of 20 units of Taq DNA Polymerase with 1 µg ΦX174 RF I DNA for 4 hours at 75°C in a 50 µl reaction resulted in < 10% conversion to RF II as determined by agarose gel electrophoresis.
Companion Products
Taq DNA Polymerase with Standard Taq Buffer
Taq 2X Master Mix
Taq DNA Polymerase with ThermoPol Buffer
Taq PCR Kit
Taq PCR Kit with Controls
Legal
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
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