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Taq 2X Master Mix |
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Prices are in US dollars and valid only for US orders.
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- Robust and reliable reactions
- Tolerates a wide range of templates
- Incorporates dUTP, dITP and fluorescently-labeled nucleotides
- Exceptional value in terms of cost per unit
Description:
Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, Standard Taq Reaction Buffer, and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
Taq DNA Polymerase is a thermostable DNA polymerase that posesses a 5´→ 3´ polymerase activity and a double-strand specific 5´→ 3´ exonuclease activity.
Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
Applications:- PCR
- Primer Extension
- Microarray Analysis
- High-Throughput PCR
Reagents Supplied:
MgCl2 (25 mM)
Taq MasterMix
Enzyme Properties
Heat Inactivation:
No
Reaction & Storage Conditions
Reaction Conditions:
1X Taq MasterMix
Incubate at
75°C.
1X Taq MasterMix:
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl2
0.2 mM dNTPs
5 % Glycerol
0.08 % NP-40
0.05 % Tween-20
25 units/ml Taq DNA Polymerase
pH 8.6 @ 25°C
Unit Definition:
One unit of Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Unit Assay Conditions: 1X Standard Taq Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Concentration:
2 X
Storage Temperature:
-20°C
Notes
General notes:- Taq 2X Master Mix is stable at 4°C for three months or for fifteen freeze-thaw cycles. For daily use, we recommend keeping an aliquot at 4°C.
- M0270S contains 125 units of Taq DNA Polymerase.
M0270L contains 625 units of Taq DNA Polymerase.
Usage notes:- Taq 2X Master Mix is to be used at a 1X concentration with DNA template and primers in a total reaction volume of 25 or 50 μl.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
5 kb Lambda PCR:
25 cycles of PCR amplification of 5 ng Lambda DNA with 2.5 units of Taq DNA Polymerase in the presence of 200 µM dNTPS, 0.2 µM primers and 1X Standard Taq Reaction Buffer resulted in a yield of 100 ng specific product.
3´→ 5´ Exonuclease Activity:
Incubation of 20 units of enzyme in 20 µl of a 10 nM solution of a fluorescent 5´- FAM labeled oligonucleotide for 30 minutes at 37°C and 75°C yields no detectable 3´→ 5´ degradation.
Endonuclease Activity:
Incubation of 20 units of Taq DNA Polymerase with 1 µg ΦX174 RF I DNA for 4 hours at 75°C in a 50 µl reaction resulted in < 10% conversion to RF II as determined by agarose gel electrophoresis.
Companion Products
Taq DNA Polymerase with Standard Taq Buffer
Taq DNA Polymerase with ThermoPol Buffer
Taq PCR Kit
Taq PCR Kit with Controls
Deoxynucleotide Solution Mix
Deoxynucleotide Solution Set
Quick-Load® Taq 2X Master Mix
Standard Taq (Mg-free) Reaction Buffer Pack
Standard Taq Reaction Buffer Pack
Legal
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
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