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FAQs for Taq DNA Polymerase with ThermoPol Buffer Protocols for Taq DNA Polymerase with ThermoPol Buffer FAQs for Polymerases Technical Reference for Polymerases Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE ThermoPol Reaction Buffer Taq DNA Polymerase with Standard Taq Buffer Taq 2X Master Mix Taq DNA Polymerase Diluent Taq PCR Kit Taq PCR Kit with Controls Deoxynucleotide Solution Mix Deoxynucleotide Solution Set Quick-Load® Taq 2X Master Mix Standard Taq (Mg-free) Reaction Buffer Pack Standard Taq Reaction Buffer Pack ThermoPol DF (Detergent-free) Reaction Buffer ThermoPol II (Mg-free) Reaction Buffer Pack

Home > Products > Polymerases > Routine PCR > Taq DNA Polymerase with ThermoPol Buffer Taq DNA Polymerase with ThermoPol Buffer Catalog # Size Concentration Price Qty   M0267L 2,000 units 5,000 units/ml $232.00 M0267S 400 units 5,000 units/ml $58.00 M0267X 4,000 units 5,000 units/ml $418.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Isolated from a recombinant source Supplied with 10X Reaction Buffer Robust and reliable reactions Tolerates a wide range of templates Incorporates dUTP, dITP and fluorescently-labeled nucleotides Exceptional value in terms of cost per unit Description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations. Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 Applications: Primer extension PCR Colony PCR Long PCR (>5 kb) Reagents Supplied: ThermoPol Reaction Buffer (10X) Enzyme Properties Polymerase Properties | Thermophilic Polymerase Characteristics 3´ to 5´ Exonuclease: No 5´ to 3´ Exonuclease: Yes Strand Displacement: No Error Rate: 285 x10-6 bases Heat Inactivation: No Molecular Weight: Theoretical: 94,000 daltons Reaction & Storage Conditions Reaction Conditions: 1X ThermoPol Reaction Buffer 1X ThermoPol Reaction Buffer: 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1 % Triton X-100 pH 8.8 @ 25°C Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. Unit Assay Conditions: 1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA. Concentration: 5,000 units/ml Storage Conditions: 10 mM Tris-HCl 100 mM KCl 1 mM Dithiothreitol 0.1 mM EDTA 50% Glycerol 0.5% Tween-20 0.5% NP-40 pH 7.4 @ 25°C Storage Temperature: -20°C FAQs Can Taq DNA Polymerase be used in other buffers? Why is the product a smear when visualized on an agarose gel? Why is there no product when visualized on an agarose gel? How should I set up a PCR reaction using Taq DNA Polymerase? The product sequence doesn't completely match the expected sequence. How can this result be improved? What type of DNA ends result from a primer extension reaction or a PCR reaction using Taq DNA Polymerase? When should Taq DNA Polymerase be used in a primer extension reaction or for PCR? Will the 5'→3' exonuclease activity of Taq DNA Polymerase degrade primers? Can Taq DNA Polymerase be used for nick translation? Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. Endonuclease Activity: Incubation of a 50 μl reaction containing 20 units of Taq DNA Polymerase with ThermoPol Buffer with 1 μg of ΦX174 RF I DNA for 4 hours at 75ºC resulted in < 10% conversion to RFII as determined by agarose gel electrophoresis. 5 kb Lambda PCR: 25 cycles of PCR amplification of 5 ng Lambda DNA with 2.5 units of Taq DNA Polymerase in the presence of 200 µM dNTPS, 0.2 µM primers and 1X Standard Taq Reaction Buffer resulted in a yield of 200 ng specific product. 3´→ 5´ Exonuclease Activity: Incubation of 20 units of Taq DNA Polymerase in 20 µl of a 10 nM solution of a fluorescent 5’-FAM labeled oligonucleotide for 30 minutes at 37°C and 75°C yields no detectable 3’-->5’ degradation as determined by high resolution denaturing PAGE. Reagents Sold Separately ThermoPol Reaction Buffer Companion Products Taq DNA Polymerase with Standard Taq Buffer Taq 2X Master Mix Taq DNA Polymerase Diluent Taq PCR Kit Taq PCR Kit with Controls Deoxynucleotide Solution Mix Deoxynucleotide Solution Set Quick-Load® Taq 2X Master Mix Standard Taq (Mg-free) Reaction Buffer Pack Standard Taq Reaction Buffer Pack ThermoPol DF (Detergent-free) Reaction Buffer ThermoPol II (Mg-free) Reaction Buffer Pack Legal Licenses/Patents/Disclaimers: Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.