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FAQs for Mung Bean Nuclease Protocols for Mung Bean Nuclease FAQs for DNA Modifying Enzymes and Cloning Technical Reference for DNA Modifying Enzymes and Cloning Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE Mung Bean Nuclease Reaction Buffer

Home > Products > DNA Modifying Enzymes and Cloning > Nucleases > Mung Bean Nuclease Mung Bean Nuclease Catalog # Size Concentration Price Qty   M0250L 7,500 units 10,000 units/ml $224.00 M0250S 1,500 units 10,000 units/ml $56.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Removal of single-stranded extensions (3'and 5') to leave ligatable blunt ends Transcriptional mapping Cleavage of hairpin loops Supplied with 10X Reaction Buffer Description: A single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from the ends of DNA and RNA molecules, leaving blunt, ligatable ends. Source: Mung bean sprouts Applications: Removal of 3´ and 5´ extensions from DNA or RNA termini Transcriptional mapping Cleavage of hairpin loops Excision of gene coding sequences from genomic DNA Generation of new restriction sites Reagents Supplied: Mung Bean Nuclease Reaction Buffer (10X) Enzyme Properties Nuclease Properties Comparison Molecular Weight: Theoretical: 39,000 daltons Reaction & Storage Conditions Reaction Conditions: 1X Mung Bean Nuclease Reaction Buffer Incubate at 30°C. 1X Mung Bean Nuclease Reaction Buffer: 50 mM sodium acetate 30 mM NaCl 1 mM ZnSO4 pH 5.0 @ 25°C Unit Definition: One unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble total nucleotide in a total reaction volume of 50 μl in 1 minute at 37°C in 1X Mung Bean Nuclease Reaction Buffer with 0.5 mg/ml denatured Calf Thymus DNA. Concentration: 10,000 units/ml Storage Conditions: 10 mM sodium acetate 0.1 mM zinc acetate 1 mM Cysteine 50% Glycerol 0.001% Triton X-100 pH 5.0 @ 25°C Storage Temperature: -20°C Notes Usage notes: It is no longer necessary to supplement Mung Bean Nuclease reactions with Zn2+. The zinc acetate in the storage buffer fulfills the Zn2+ requirement of the enzyme even after dilution in a reaction. FAQs Should I use Mung Bean Nuclease or DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210)? Is the Mung Bean Nuclease active in other NEBuffers? Can Mung Bean Nuclease be heat inactivated? Does Zn2+ need to be added to a Mung Bean Nuclease reaction? Will Mung Bean Nuclease degrade double stranded DNA? Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. Quality Assurance Statement: Purified free of double-strand exonuclease contamination. Terminal Integrity: After incubation of 1 μg of lambda DNA (Hae III digest) with 10 units of Mung Bean Nuclease at 30ºC for 30 hour(s), > 95% of the DNA fragments can be ligated with T4 DNA ligase. Of these ligated fragments, >95% can be recut with Hae III. A) Molecular weight standard (λ HindIII digest) B) M13mp8 (supercoiled) C) As in B after incubation with NcoI D) As in B after incubation with HindIII E) As in D after incubation with Mung Bean Nuclease F) As in E after incubation with T4 DNA Ligase G) As in F after incubation with NcoI H) As in F after incubation with HindIII References Kowalski, D. et al. (1976) Biochemistry, 15, 4457-4463. McCutchan, T.F. et al. (1984) Science, 225, 626-628. Reagents Sold Separately Mung Bean Nuclease Reaction Buffer