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Epigenetics >
Enzymes >
PRMT1 Methyltransferase |
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Prices are in US dollars and valid only for US orders.
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Description:
PRMT1 is a major protein arginine methyltransferase (1). It specifically methylates arginine 3 (Arg 3) of H4 in vitro and in vivo. Furthermore, methylation of histone H4 at Arg 3 facilitates transcriptional activation by nuclear hormone receptor (2). Ordered cooperative functions of PRMT1, p300 and CARM1 in transcriptional activation by p53 is observed on the GADD45 gene following ectopic p53 expression and/or UV irradiation (3).
Source:
PRMT1 enzyme is expressed from rat PMRT1 cDNA using an E. coli GST fusion expression system.
Reagents Supplied:
HMT Reaction Buffer (10X)
S-adenosylmethionine (SAM) (32 mM)
Properties & Usage
Heat Inactivation:
65°C for 20 minutes
Reaction & Storage Conditions
Reaction Conditions:
1X HMT Reaction Buffer
Supplemented with 160 μM S-adenosylmethionine (SAM)
Incubate at
37°C.
1X HMT Reaction Buffer:
50 mM Tris-HCl
5 mM MgCl2
4 mM Dithiothreitol
pH 9.0 @ 25°C
Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the transfer of 1 pmol of methyl group to synthetic peptide substrate representing the first 17 amino acids of histone H4 in a total reaction volume of 25 µl in 10 minutes at 37°C.
Concentration:
2,000 units/ml
Storage Conditions:
50 mM Tris-HCl
100 mM NaCl
5 mM MgCl2
4 mM Dithiothreitol
50% Glycerol
pH 8.0 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- Storage Note: S-adenosylmethionine (SAM) is stored at -20°C as a 32 mM solution dissolved in 0.005 M sulfuric acid and 10% ethanol (pH 7.5). Under these conditions, SAM is stable for up to 6 months. SAM is unstable at 37°C and should be replenished in reactions incubated longer than 4 hours. Methylation can be optimized by using fresh SAM.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
Purified free of contaminating proteases.
References
- Tang, J. et al. (2000) J. Biol. Chem., 275, 7723-7730.
- Wang, H. et al. (2001) Science, 293, 853-857.
- An, W. et al. (2004) Cell, 117, 735-748.
Reagents Sold Separately
S-adenosylmethionine (SAM)
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