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Enzymes >
SET7 Methyltransferase |
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Description:
SET7 Methyltransferase methylates lysine 4 (Lys 4) of histone H3 (1). Methylation occurs at the e amino group of lysine residues (2,3). Di- and tri- methylation of histone H3 Lys 4 is a hallmark of transcriptionally active chromatin and is globally distributed (4,5).
Source:
SET7 enzyme is expressed from human SET7 cDNA using a baculovirus expression system using an intein-CBD fusion tag.
Reagents Supplied:
HMT Reaction Buffer (10X)
S-adenosylmethionine (SAM) (32 mM)
Enzyme Properties
Heat Inactivation:
65°C for 20 minutes
Reaction & Storage Conditions
Reaction Conditions:
1X HMT Reaction Buffer
Supplemented with 160 μM S-adenosylmethionine (SAM)
Incubate at
37°C.
1X HMT Reaction Buffer:
50 mM Tris-HCl
5 mM MgCl2
4 mM Dithiothreitol
pH 9.0 @ 25°C
Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the transfer of 1 pmol of methyl group to synthetic peptide substrate representing the first 17 amino acids of histone H3 in a total reaction volume of 25 µl in 10 minutes at 37°C.
Concentration:
2,000 units/ml
Storage Conditions:
50 mM Tris-HCl
100 mM NaCl
5 mM MgCl2
4 mM Dithiothreitol
50% Glycerol
pH 8.0 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:- Storage Note: S-adenosylmethionine (SAM) is stored at -20°C as a 32 mM stolution dissolved in 0.005 M sulfuric acid and 10% ethanol (pH 7.5). Under these conditions, SAM is stable for up to 6 months. SAM is unstable at 37°C and should be replenished in reactions incubated longer than 4 hours. Methylation can be optimized by using fresh SAM.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
Purified free of contaminating proteases.
References
- Wang, H. et al. (2001) Mol Cell, 6, 1207-1217.
- Xiao, B. et al. (2003) Nature, 421, 652-656.
- Wilson, J.R. et al. (2002) Cell, 111, 105-115.
- Schneider, R. et al. (2004) Nat. Cell Biol., 6, 73-77.
- Santos-Rosa, H. et al. (2002) Nature, 419, 407-411.
Reagents Sold Separately
S-adenosylmethionine (SAM)
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