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FAQs for Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab FAQs for Epigenetics Technical Reference for Epigenetics Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE Human DNA (cytosine-5) Methyltransferase (Dnmt1)

Home > Products > Epigenetics > Antibodies > Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab Catalog # Size Concentration Price Qty   M0231L 0.15 ml   $600.00 M0231S 0.05 ml   $250.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Description: Amino terminal (N-terminal) of human Dnmt1 has been shown to bind proliferating cell nuclear antigen (PCNA) and histone deactylase (1,2). Deletion of this region stimulates DNA methylation (3). In neoplastic transformed cells methylation pattern is abnormal, leading to the speculation that Dnmt1 may be involved in such processes (4). Sensitivity: Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Antibody detects less than 10 ng of Dnmt1 in the cell extract using anti-rabbit Phototope-HRP Western Detection Kit (available from Cell Signaling Technology). Dnmt1 Amino-terminal Antibody: Polyclonal antibodies are produced by immunizing rabbit with a KLH coupled synthetic peptide corresponding to residues in the N-terminal region of human Dnmt1. The antibodies are purified in a protein A column. Figure 1: Western analysis of cell extracts; Lane 1: Sf9 cells, Lane 2: Human DNA (cytosine-5) Methyltransferase expressing Sf9 cells, Lane 3: 293 cells, Lane 4: Hela cells, Lane 5: Jurkat cells, Lane 6: COS cells, Lane 7: Mouse DNA (cytosine-5) Methyltransferase expressing Sf9 cells, using Human DNA (cytosine-5) Methyltransferase Amino-terminal Antibody. Specificity/Cross-reactivity: human, monkey Antibody Application Recommended Dilution Sensitivity Detection Method Western Blot 1:2000 10 ng Western Blot Storage Conditions Storage Conditions: 10 mM HEPES 150 mM NaCl 100 µg/ml BSA 50% Glycerol pH 7.5 @ 25°C Storage Temperature: -20°C FAQs What dilution should be used with Dnmt1 Amino-terminal Ab? How much protein should be run on the gel for detection with Dnmt1 Amino-terminal Ab? How much of NEB Dnmt1 should be used as a control when using Dnmt1 Amino-terminal Ab? What is the species cross reactivity of Dnmt1 Amino-terminal Ab? Can Dnmt1 Amino-terminal Ab be used to detect mouse and rat? References Chuang, L.S. et al. (1997) Science, 277, 1996-2000. Fuks, F. et al. (2000) Nature Genet., 24, 88-91. Bestor, T.H. (1992) EMBO J., 11, 2611-2617. Schmutte, C. et al. (1998) Biol. Chem., 379, 377-388. Companion Products Human DNA (cytosine-5) Methyltransferase (Dnmt1)