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FAQs for Phusion™ High-Fidelity PCR Kit Protocols for Phusion™ High-Fidelity PCR Kit FAQs for Polymerases Technical Reference for Polymerases Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE Phusion™ GC Buffer Pack Phusion™ HF Buffer Pack Phusion™ High-Fidelity DNA Polymerase Deoxynucleotide Solution Mix

Home > Products > Polymerases > PCR Kit > Phusion™ High-Fidelity PCR Kit Phusion™ High-Fidelity PCR Kit Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. Catalog # Size Concentration Price Qty   F-553L 200 reactions (50 μl vol)   $210.00 F-553S 50 reactions (50 μl vol)   $62.00 Prices are in US dollars and valid only for US orders. Download: Manual|MSDS PDF Licensed for PCR* Highest fidelity of any commercial thermostable polymerase (50X greater than Taq) Industry leading processivity is 10X higher than Pfu and 2X higher than Taq Unrivalled robustness and yield with minimal amounts of enzyme and little optimization Description: Phusion High-Fidelity PCR Kit is available in two sizes. The small kit (F-553S) contains all the necessary reagents for performing 50 amplifications in 50 µl volume or 125 amplifications in 20 µl volume. The large kit contains reagents for 200 amplifications in 50 µl volume or 500 amplifications in 20 µl volume. The kit also contains control template (lambda DNA) and two primer sets (1.3 kb and 10 kb) for performing 20 control reactions in 50 µl volume or 50 control reactions in 20 µl volume. Kit Components: Phusion™ GC Buffer Pack (5X) Phusion™ HF Buffer Pack (5X) Phusion™ High-Fidelity DNA Polymerase (2,000 units/ml) 1.3 kb primers (4 μM each) 10 kb primers (4 μM each) Control lambda template (0.5 ng/μl) Deoxynucleotide Solution Mix (10 mM) DMSO (100 %) DNA Size Standard (λ DNA HindIII + ΦX174 DNA HaeIII digests) (50 ng/μl) MgCl2 solution (50 mM) Storage Conditions Storage Temperature: -20°C Stable for at least one year from the packaging date. FAQs Are the DNA fragments produced by Phusion™ High-Fidelity DNA Polymerase/Phusion™ High-Fidelity PCR Master Mix blunt-ended or do they have the single-base 3´ overhang that Taq DNA Polymerase yields? My template is GC rich or supercoiled. How can I optimize my product yield using Phusion™ High-Fidelity DNA Polymerase? What are the advantages to using Phusion™ High-Fidelity DNA Polymerase? What is the error rate of Phusion™ High-Fidelity DNA Polymerase? I am having trouble amplifying a template that is longer than 5kb. How can I optimize my product yield using Phusion™ High-Fidelity DNA Polymerase? Why do I see no product or low yield on an agarose gel after a PCR using Phusion™ High-Fidelity DNA Polymerase? Why are there high molecular weight smears or DNA in the wells of an agarose gel after a PCR using Phusion™ High-Fidelity DNA Polymerase? Will Phusion™ High-Fidelity DNA Polymerase incorporate dUTPs? Why are there low molecular weight discrete bands on an agarose gel after a PCR using Phusion™ High-Fidelity DNA Polymerase? I'd like to clone a fragment amplified with Phusion™ High-Fidelity DNA Polymerase/Phusion™ High-Fidelity PCR Master Mix. Do I have to blunt end clone? What should my primer concentration be when using Phusion™ High-Fidelity DNA Polymerase/Phusion™ High-Fideltiy PCR Master Mix? Does Phusion High-Fidelity DNA Polymerase exhibit a strand displacement activity? Are Finnzymes' DNA Polymerases licensed for PCR? Reagents Sold Separately Phusion™ GC Buffer Pack Phusion™ HF Buffer Pack Phusion™ High-Fidelity DNA Polymerase Deoxynucleotide Solution Mix Legal Licenses/Patents/Disclaimers: * PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD, Roche Molecular Systems, Inc. and Applied Biosystems. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler. Notice to purchaser: Limited license. The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by New England Biolabs, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.