To access your account, log in or register.	shopping cart | log in

FAQs for DyNAzyme™ EXT PCR Kit Protocols for DyNAzyme™ EXT PCR Kit FAQs for Polymerases Technical Reference for Polymerases Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE DyNAzyme™ EXT DNA Polymerase DyNAzyme™ EXT Buffer Pack Optimized Detergent-free DyNAzyme™ EXT Buffer Optimized DyNAzyme™ EXT Buffer and 50 mM MgCl2

Home > Products > Polymerases > PCR Kit > DyNAzyme™ EXT PCR Kit DyNAzyme™ EXT PCR Kit Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. Catalog # Size Concentration Price Qty   F-552L 1,000 reactions (50 μl vol)   $495.00 F-552S 200 reactions (50 μl vol)   $125.00 Prices are in US dollars and valid only for US orders. Download: Manual|MSDS PDF Licensed for PCR* Exceptional Performance - masters demanding reaction conditions without time consuming optimization Excellent for long templates - amplifies up to 40 kb Ideal for amplifying difficult templates, such as GC-rich or looped sequences Description: DyNAzyme EXT DNA Polymerase Kit is designed to provide superior PCR amplifications for long and difficult PCR and for all standard amplifications. Using the control template included, 20 amplifications can be performed with the 500 bp control template and primers or with the 20 kb control template and primers. Kit Components: dNTP Mix (10 mM each nt) DyNAzyme™ EXT DNA Polymerase (1,000 units/ml) 20 kb primers (25 μM) 500 bp primers (25 μM) Control λ DNA (0.5 ng/μl) DNA Size Standard (λ DNA HindIII + ΦX174 DNA HaeIII digests) (50 ng/μl) Optimized DyNAzyme™ EXT Buffer (Mg2+-free buffer also supplied) DMSO (100 %) Gel Loading Dye MgCl2 solution (50 mM) Storage Conditions Storage Temperature: -20°C Stable for at least one year. FAQs Can I use DyNAzyme EXT DNA Polymerase to PCR directly from bacterial colonies? Does DyNAzyme EXT DNA Polymerase add the non-template dependent 3'-A overhang? How can I amplify templates with hairpin-loop structures or high GC content using DyNAzyme EXT DNA Polymerase? What applications should DyNAzyme EXT DNA Polymerase be used for? What are the advantages of using DyNAzyme EXT DNA Polymerase? I need very high fidelity, what reaction conditions should I use with DyNAzyme EXT DNA Polymerase? I see foaming when my DyNAzyme EXT DNA Polymerase amplified PCR products are spotted on microarray slides. What causes the smearing of the whole lane upon gel electrophoresis after PCR with DyNAzyme EXT DNA Polymerase? I can't get DyNAzyme EXT DNA Polymerase to work yet Taq DNA Polymerase works fine. Does DMSO inhibit DyNAzyme EXT Polymerase? How can I dilute DyNAzyme EXT DNA Polymerase? When we received our shipment the ice had melted and the DyNAzyme EXT DNA Polymerase was at room temperature. Will it be all right to use? Are Finnzymes' DNA Polymerases licensed for PCR? Is DyNAzyme EXT DNA Polymerase contaminated with DNA? What nucleotide analogues can I use with DyNAzyme EXT DNA Polymerase? Reagents Sold Separately DyNAzyme™ EXT DNA Polymerase Companion Products DyNAzyme™ EXT Buffer Pack Optimized Detergent-free DyNAzyme™ EXT Buffer Optimized DyNAzyme™ EXT Buffer and 50 mM MgCl2 Legal Licenses/Patents/Disclaimers: * PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.