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Phusion™ Flash High-Fidelity PCR Master Mix
Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc.
Recombinant Source
Catalog # Size Concentration Price Qty  
F-548L 500 reactions (20 μl vol)   $300.00
F-548S 100 reactions (20 μl vol)   $75.00
Prices are in US dollars and valid only for US orders.
Download:Manual|MSDS PDF


Description:
Phusion™ Flash High-Fidelity PCR Master Mix is a novel reagent developed especially for fast PCR. It utilizes modified Phusion Hot Start High-Fidelity DNA Polymerase, which ensures the accuracy of the DNA amplification. With this reagent, both low and high complexity DNA can be amplified with a PCR protocol using an extension step of only 15 seconds or less per kilobase. The robustness of Phusion Flash PCR Master Mix minimizes the need for optimization. 

Phusion Flash PCR Master Mix was developed to save valuable laboratory time. First, use of Master Mix reduces required pipetting steps at reaction setup. Second, the unique composition of the Phusion Flash PCR Master Mix enables the use of extremely short cycling protocols in all PCR instruments.











Source:
Isolated and purified from an E. coli strain carrying a plasmid with the cloned Phusion DNA Polymerase gene.

The Affibody ligand is isolated and purified from an E. coli strain carrying a plasmid with the cloned Affibody encoding gene.

Advantages:
  • Extreme speed - extension times of 15 s/kb or less
  • Accuracy - proofreading DNA polymerase with a fidelity of 25 x Taq
  • Yield - high yields in reduced time

Reaction & Storage Conditions


Storage Temperature:
-20°C

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