Description: Phusion® Flash High-Fidelity PCR Master Mix is a novel reagent developed especially for fast PCR. It utilizes modified Phusion Hot Start II High-Fidelity DNA Polymerase, which ensures the accuracy of the DNA amplification. With this reagent, both low and high complexity DNA can be amplified with a PCR protocol using an extension step of only 15 seconds or less per kilobase. The robustness of Phusion Flash PCR Master Mix minimizes the need for optimization.
Phusion Flash PCR Master Mix was developed to save valuable laboratory time. First, use of Master Mix reduces required pipetting steps at reaction setup. Second, the unique composition of the Phusion Flash PCR Master Mix enables the use of extremely short cycling protocols in all PCR instruments.
A 1.5 kb human cathepsin K gene was amplified with three different DNA polymerases according to supplier's recommendations. Varying extension times (10-60 seconds) were used in a two-step PCR protocol. Total PCR cycling times were compared between three different DNA polymerases using either Finnzymes' Piko Thermal Cycler (fast cycler) or the Tetrad Thermal Cycler from Bio-Rad. A 1.5 kb human cathepsin K gene was amplified according to supplier's recommendations. Source: Isolated and purified from an E. coli strain carrying a plasmid with the cloned Phusion DNA Polymerase gene.
The Affibody ligand is isolated and purified from an E. coli strain carrying a plasmid with the cloned Affibody encoding gene.
Advantages:
Extreme speed - extension times of 15 s/kb or less
Accuracy - proofreading DNA polymerase with a fidelity of 25 x Taq
