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FAQs for Phusion™ High-Fidelity PCR Master Mix with GC Buffer Protocols for Phusion™ High-Fidelity PCR Master Mix with GC Buffer FAQs for Polymerases Technical Reference for Polymerases Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE Phusion™ GC Buffer Pack Phusion™ High-Fidelity DNA Polymerase

Home > Products > Polymerases > Master Mixes > Phusion™ High-Fidelity PCR Master Mix with GC Buffer Phusion™ High-Fidelity PCR Master Mix with GC Buffer Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. Catalog # Size Concentration Price Qty   F-532L 500 reactions (50 μl vol)   $650.00 F-532S 100 reactions (50 μl vol)   $162.00 Prices are in US dollars and valid only for US orders. Download: Manual|MSDS PDF Licensed for PCR* Highest fidelity of any commercial thermostable polymerase (50 X greater than Taq) Industry leading processivity is 10 X higher than Pfu and 2 X higher than Taq Unrivalled robustness and yield with minimal amounts of enzyme and little optimization Convenient 2X mix - add only template and primers Description: Phusion High-Fidelity PCR Master Mix is a convenient 2X mix containing Phusion High-Fidelity DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user. Choosing the right master mix: The error rate of Phusion High-Fidelity DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the master mix with HF Buffer should be used as a default for high fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, i.e. GC rich templates or those with complex secondary structures. Use of the Phusion Master Mix with GC Buffer is recommended for those instances where amplification with HF Buffer has failed. Source: Isolated and purified from an E. coli strain carrying a plasmid with the cloned Phusion DNA Polymerase gene. The Affibody ligand is isolated and purified from an E. coli strain carrying a plasmid with the cloned Affibody encoding gene. Reagents Supplied: DMSO (100 %) Reaction & Storage Conditions Reaction Conditions: 1X Phusion High-Fidelity PCR Master Mix with GC Buffer 1X Phusion High-Fidelity PCR Master Mix with GC Buffer: 0.2 mM dNTPs 1 X Phusion GC Buffer 1 X Phusion High-Fidelity DNA Polymerase Storage Temperature: -20°C Notes General notes: The Phusion GC Buffer supplies 1.5 mM MgCl2 in a final reaction concentration. Stable for six months from the packaging date. After thawing the mix can be refrozen or optionally stored at 4°C for three months. FAQs Are the DNA fragments produced by Phusion™ High-Fidelity DNA Polymerase/Phusion™ High-Fidelity PCR Master Mix blunt-ended or do they have the single-base 3´ overhang that Taq DNA Polymerase yields? What is the error rate of Phusion™ High-Fidelity DNA Polymerase? What are the advantages to using Phusion™ High-Fidelity PCR Master Mixes? I am having trouble amplifying a template that is longer than 5kb. How can I optimize my product yield using Phusion™ High-Fidelity PCR Master Mix? I am having trouble amplifying a template that is longer than 5kb. How can I optimize my product yield using Phusion™ High-Fidelity PCR Master Mix? Why are there high molecular weight smears or DNA in the wells of an agarose gel after a PCR using Phusion™ High-Fidelity DNA Polymerase? Why are there low molecular weight discrete bands on an agarose gel after a PCR using Phusion™ High-Fidelity PCR Master Mix? Does Phusion High-Fidelity DNA Polymerase exhibit a strand displacement activity? What should my primer concentration be when using Phusion™ High-Fidelity DNA Polymerase/Phusion™ High-Fideltiy PCR Master Mix? Will Phusion™ High-Fidelity DNA Polymerase incorporate dUTPs? I'd like to clone a fragment amplified with Phusion™ High-Fidelity DNA Polymerase/Phusion™ High-Fidelity PCR Master Mix. Do I have to blunt end clone? Are Finnzymes' DNA Polymerases licensed for PCR? Companion Products Phusion™ GC Buffer Pack Phusion™ High-Fidelity DNA Polymerase Legal Licenses/Patents/Disclaimers: * PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD, Roche Molecular Systems, Inc. and Applied Biosystems. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler. Notice to purchaser: Limited license. The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by New England Biolabs, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.