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FAQs for Phusion® HF Buffer Pack FAQs for Polymerases and Amplification Technical Reference for Polymerases and Amplification Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE Detergent-free Phusion® HF Buffer Pack Phusion® High-Fidelity DNA Polymerase Phusion® High-Fidelity PCR Kit Phusion® Hot Start High-Fidelity DNA Polymerase

Home > Products > Polymerases and Amplification > Reaction Buffers and Diluents > Phusion® HF Buffer Pack Phusion® HF Buffer Pack Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. Catalog # Size Concentration Price Qty   F-518L 6 ml   $14.00 F-518S 3 ml   $9.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Licensed for PCR* Description: The error rate of Phusion® High-Fidelity DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as a default for high fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, i.e. GC rich templates or those with complex secondary structures. Use of the GC Buffer is recommended for those instances where amplification with HF Buffer has failed. The detergent-free buffers for Phusion DNA Polymerase are designed for applications like DHPLC where PCR products are recommended to be free of detergents. They can also be used in microarray applications, as detergents can cause foaming when PCR products are spotted on microarray slides. Kit Components: Phusion™ HF Reaction Buffer (5X) DMSO (100 %) MgCl2 solution (50 mM) Storage Conditions Storage Temperature: -20°C Notes General notes: 1X Buffer provides 1.5 mM MgCl2. FAQs Should I use HF Buffer or GC Buffer with Phusion™ High-Fidelity DNA Polymerase? When should I use the Detergent-free HF or GC Buffer? What is supplied with the Phusion™ HF Buffer Pack? What are the error rates of Phusion™ High-Fidelity DNA Polymerase in HF and GC buffers? Is Phusion HF or Phusion GC buffer stable at room temperature? Are the MgCl2 and DMSO supplied with the HF and GC Buffers? Companion Products Detergent-free Phusion® HF Buffer Pack Phusion® High-Fidelity DNA Polymerase Phusion® High-Fidelity PCR Kit Phusion® Hot Start High-Fidelity DNA Polymerase Legal Licenses/Patents/Disclaimers: * PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD, Roche Molecular Systems, Inc. and Applied Biosystems. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler. Notice to purchaser: Limited license. The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by New England Biolabs, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201