Exceptional Performance - masters demanding reaction conditions without time consuming optimization
Increased Specificity - prevents formation of primer-dimers and non-specific amplification
Allows room temperature reaction set up
Description: Finnzymes' DyNAzyme™ II Hot Start DNA Polymerase is a modified form of the DyNAzyme II DNA Polymerase originating from Thermus brockianus. DyNAzyme II Hot Start DNA Polymerase is inactive at room temperature, and the activity is recovered by a 10-minute incubation step at 94°C. The enzyme improves the specificity of PCR by preventing the extension of non-specifically bound primers during reaction setup and the first heating cycle. The use of DyNAzyme II Hot Start DNA Polymerase is also beneficial when using primers that are prone to primer-dimer formation or when using robotic PCR systems that include incubations at room temperature prior to temperature cycling. DyNAzyme II Hot Start DNA Polymerase possesses both 5´->3´ DNA polymerase and 5´->3´ exonuclease activities. The polymerase amplifies DNA targets up to 3 kb, but optimal results are obtained with amplicons up to 1 kb.
Source: Isolated and purified from an E. coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus.
Advantages:
Increased specificity
Prevents the formation of primer-dimers
Reaction setup can be done at room temperature
Sensitivity
Incorporates dUTP and dITP
Reagents Supplied: DyNAzyme™ II Hot Start Reaction Buffer (10X)
Reaction & Storage Conditions Reaction Conditions: 1X DyNAzyme™ II Hot Start Reaction Buffer
1X DyNAzyme™ II Hot Start Reaction Buffer: 15 mM Tris-HCl 30 mM KCl 5 mM (NH4)2SO4 2.5 mM MgCl2 0.02 % BSA
pH 8.2 @ 25°C
Concentration: 2 units/μl
Storage Conditions: 20 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.5% Tween-20
pH 9.0 @ 25°C
Legal Licenses/Patents/Disclaimers: * PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.
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