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FAQs for DyNAzyme™ II Hot Start DNA Polymerase Protocols for DyNAzyme™ II Hot Start DNA Polymerase FAQs for Polymerases Technical Reference for Polymerases Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Polymerases > Hot Start PCR > DyNAzyme™ II Hot Start DNA Polymerase DyNAzyme™ II Hot Start DNA Polymerase Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. Catalog # Size Concentration Price Qty   F-504L 1,000 units 2 units/μl $440.00 F-504S 250 units 2 units/μl $130.00 Prices are in US dollars and valid only for US orders. Download: Manual|MSDS PDF Licensed for PCR* Exceptional Performance - masters demanding reaction conditions without time consuming optimization Increased Specificity - prevents formation of primer-dimers and non-specific amplification Allows room temperature reaction set up Description: Finnzymes' DyNAzyme™ II Hot Start DNA Polymerase is a modified form of the DyNAzyme II DNA Polymerase originating from Thermus brockianus. DyNAzyme II Hot Start DNA Polymerase is inactive at room temperature, and the activity is recovered by a 10-minute incubation step at 94°C. The enzyme improves the specificity of PCR by preventing the extension of non-specifically bound primers during reaction setup and the first heating cycle. The use of DyNAzyme II Hot Start DNA Polymerase is also beneficial when using primers that are prone to primer-dimer formation or when using robotic PCR systems that include incubations at room temperature prior to temperature cycling. DyNAzyme II Hot Start DNA Polymerase possesses both 5´->3´ DNA polymerase and 5´->3´ exonuclease activities. The polymerase amplifies DNA targets up to 3 kb, but optimal results are obtained with amplicons up to 1 kb. Source: Isolated and purified from an E. coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus. Advantages: Increased specificity Prevents the formation of primer-dimers Reaction setup can be done at room temperature Sensitivity Incorporates dUTP and dITP Reagents Supplied: DyNAzyme II Hot Start DNA Polymerase Reaction Buffer (10X) Reaction & Storage Conditions Reaction Conditions: 1X DyNAzyme II Hot Start DNA Polymerase Reaction Buffer 1X DyNAzyme II Hot Start DNA Polymerase Reaction Buffer: 15 mM Tris-HCl 30 mM KCl 5 mM (NH4)2SO4 2.5 mM MgCl2 0.02 % BSA pH 8.2 @ 25°C Concentration: 2 units/μl Storage Conditions: 20 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.5% Tween-20 pH 9.0 @ 25°C Storage Temperature: -20°C FAQs What applications should DyNAzyme II Hot Start DNA Polymerase be used for? I have no product or low yield after amplification with DyNAzyme II Hot Start DNA Polymerase. What can I do if there are low molecular weight discrete bands in my PCR product after amplication with DyNAzyme II Hot Start DNA Polymerase? What should I do if there are high molecular weight smears in my PCR product when using DyNAzyme II Hot Start DNA Polymerase? Are Finnzymes' DNA Polymerases licensed for PCR? At what temperature should DyNAzyme II Hot Start DNA Polymerase be stored? Legal Licenses/Patents/Disclaimers: * PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.