 |
|
 |
|
|
| Home >
Products >
Polymerases >
RT-PCR and qRT-PCR >
DyNAmo™ cDNA Synthesis Kit |
 | | | | DyNAmo™ cDNA Synthesis Kit |
| | | Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. | |
| | |
|
|
|
 |
|
Prices are in US dollars and valid only for US orders.
|
Description:
The DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications, where amplicons are usually around 100 bp in length. This kit is a part of Finnzymes' DyNAmo 2-step qRT-PCR Kits and it can be used in conjunction with DyNAmo qPCR Kits or with any other qPCR kit suitable for the application.
The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis to be used in qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed RNA can be used as a template for reverse transcription. The kit includes both random primers and oligo(dT) primers. The user can choose either of these or alternatively use gene-specific primers.The reverse transcriptase in the kit is M-MuLV RNase H+, which provides higher sensitivity to qPCR than RNase H- reverse transcriptases.
Advantages:- Broad dynamic range: up to 1 µg of RNA
- Reliable results with different RNA sources: mRNA, total RNA, viral RNA and in vitro transcribed RNA
- Flexible: all priming options for cDNA synthesis
- Optimized 2x mix for cDNA synthesis
- Convenient to use: short protocol
Kit Components:
M-MuLV RNase H+ Reverse Transcriptase (includes RNase Inhibitor)
Oligo(dT)15 primer (100 ng/μl)
Random Hexamers (300 ng/μl)
RT Reaction Buffer (2 X)
Storage Conditions
Storage Temperature:
-20°C
Notes
General notes:- The DyNAmo cDNA Synthesis Kit is shipped in gel ice. Upon arrival, store all kit components at -20 °C. All the kit components can be refrozen and stored at -20 °C without affecting the performance of the kit.
- The kit is stable for one year from the date of packaging when stored and handled properly.
FAQs
- Are Finnzymes' DNA Polymerases licensed for PCR?
Companion Products
DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ Capillary SYBR® Green qPCR Kit
DyNAmo™ Flash Probe qPCR Kit
DyNAmo™ HS SYBR® Green qPCR Kit
DyNAmo™ Probe 2-Step qRT-PCR Kit
DyNAmo™ Probe qPCR Kit
DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ SYBR® Green qPCR Kit
DyNAmo™ SYBR® Green qPCR Kit With ROX™ Passive Reference Dye
Legal
Licenses/Patents/Disclaimers:
PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.
Notice to purchaser: Limited license. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. Use of DyNAmo Probe qPCR Kits may also require licenses from other third parties such as, but not limited to, the owners of hybridization probes and molecular beacons. Purchase of DyNAmo Probe qPCR Kits does not provide such licenses and further information on purchasing licenses to practice this technology must be obtained from the appropriate intellectual property owners of such technology.
|
|
|
 |
|
|
