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DyNAmo™ Flash Probe qPCR Kit |
 | | | | DyNAmo™ Flash Probe qPCR Kit |
| | | Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. | |
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Prices are in US dollars and valid only for US orders.
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Description:
DyNAmo™ Flash Probe qPCR Kit is the perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. Performance of the DyNAmo Flash Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase. Sensitivity and the excellent amplification efficiency across a wide range of template concentrations guarantee reproducible results.
DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillary-based realtime PCR instruments.
Request a qPCR booklet
Figure 1: Time savings with both fast and conventional real-time PCR instruments: 13 probe-based qPCR kits from 10 suppliers were tested according to suppliers’ recommendations. DyNAmo Flash Probe qPCR Kit delivers results faster than any other kit tested. Similar time savings are achieved with DyNAmo Flash qPCR Kit for SYBR Green chemistry.
Figure 2: Efficient amplification across a broad range of template concentrations: Amplification of a 118 bp amplicon from Salmonella typhimurium InvA gene with DyNAmo Flash Probe qPCR Kit (A: combined annealing and extension 15 s, total run time 67 min) and a kit from a major supplier (B: combined annealing and extension 1 min, total run time 106 min). Reaction setup and cycling was performed as recommended by the suppliers. The results show that DyNAmo Flash Probe qPCR Kit delivers higher amplification efficiency with shorter analysis time.
Advantages:- Extremely fast protocols: combined annealing and extension step of only 15 s
- Specific and sensitive detection of a wide range of template concentrations
- Included dUTP allows the use of UNG for prevention of carry-over contamination
- Convenient 2x master mix and optimized protocols
- Validated with all major real-time PCR instruments
- Licenced for qPCR
Kit Components:
Master Mix (2X)
ROX Passive Reference Dye (50X)
Storage Conditions
Storage Temperature:
-20°C
FAQs
- How is absolute quantification performed?
- How do i determine the ratio between the quantity of a target molecule in a sample and in the calibrator (healthy tissue or untreated cells, for example)?
Protocols
Protocols for DyNAmo™ Flash Probe qPCR Kit
Companion Products
DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ Capillary SYBR® Green qPCR Kit
DyNAmo™ cDNA Synthesis Kit
DyNAmo™ Flash SYBR® Green qPCR Kit
DyNAmo™ HS SYBR® Green qPCR Kit
DyNAmo™ Probe 2-Step qRT-PCR Kit
DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ SYBR® Green qPCR Kit
DyNAmo™ SYBR® Green qPCR Kit With ROX™ Passive Reference Dye
Legal
Licenses/Patents/Disclaimers:
PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.
Notice to purchaser: Limited license. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. Use of DyNAmo Probe qPCR Kits may also require licenses from other third parties such as, but not limited to, the owners of hybridization probes and molecular beacons. Purchase of DyNAmo Probe qPCR Kits does not provide such licenses and further information on purchasing licenses to practice this technology must be obtained from the appropriate intellectual property owners of such technology.
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New
England Biolabs, Inc. is an ISO 9001 and ISO
14001 Certified Company.
NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201 |
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