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Protocols for DyNAmo™ Flash Probe qPCR Kit FAQs for Polymerases Technical Reference for Polymerases Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit DyNAmo™ Capillary SYBR® Green qPCR Kit DyNAmo™ cDNA Synthesis Kit DyNAmo™ Flash SYBR® Green qPCR Kit DyNAmo™ HS SYBR® Green qPCR Kit DyNAmo™ Probe 2-Step qRT-PCR Kit DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit DyNAmo™ SYBR® Green qPCR Kit DyNAmo™ SYBR® Green qPCR Kit With ROX™ Passive Reference Dye

Home > Products > Polymerases > qPCR Products > DyNAmo™ Flash Probe qPCR Kit DyNAmo™ Flash Probe qPCR Kit New | Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. Catalog # Size Concentration Price Qty   F-455L 200 reactions   $315.00 F-455S 40 reactions   $78.00 Prices are in US dollars and valid only for US orders. Download: Manual|MSDS PDF Description: DyNAmo™ Flash Probe qPCR Kit is the perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. Performance of the DyNAmo Flash Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase. Sensitivity and the excellent amplification efficiency across a wide range of template concentrations guarantee reproducible results. DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillary-based realtime PCR instruments. Figure 1: Time savings with both fast and conventional real-time PCR instruments: 13 probe-based qPCR kits from 10 suppliers were tested according to suppliers’ recommendations. DyNAmo Flash Probe qPCR Kit delivers results faster than any other kit tested. Similar time savings are achieved with DyNAmo Flash qPCR Kit for SYBR Green chemistry. Figure 2: Efficient amplification across a broad range of template concentrations: Amplification of a 118 bp amplicon from Salmonella typhimurium InvA gene with DyNAmo Flash Probe qPCR Kit (A: combined annealing and extension 15 s, total run time 67 min) and a kit from a major supplier (B: combined annealing and extension 1 min, total run time 106 min). Reaction setup and cycling was performed as recommended by the suppliers. The results show that DyNAmo Flash Probe qPCR Kit delivers higher amplification efficiency with shorter analysis time. Advantages: Extremely fast protocols: combined annealing and extension step of only 15 s Specific and sensitive detection of a wide range of template concentrations Included dUTP allows the use of UNG for prevention of carry-over contamination Convenient 2x master mix and optimized protocols Validated with all major real-time PCR instruments Licenced for qPCR Kit Components: Master Mix (2X) ROX Passive Reference Dye (50X) Storage Conditions Storage Temperature: -20°C Companion Products DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit DyNAmo™ Capillary SYBR® Green qPCR Kit DyNAmo™ cDNA Synthesis Kit DyNAmo™ Flash SYBR® Green qPCR Kit DyNAmo™ HS SYBR® Green qPCR Kit DyNAmo™ Probe 2-Step qRT-PCR Kit DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit DyNAmo™ SYBR® Green qPCR Kit DyNAmo™ SYBR® Green qPCR Kit With ROX™ Passive Reference Dye